UID:
almafu_9961427392502883
Format:
1 videorecording (42 min., 50 sec.) :
,
sound, color
,
004250
Content:
Audio-visual presentation: Blood-brain barrier -- In vitro models of blood-brain barrier -- Blood-brain barrier permeability -- Brain endothelium and tight junctions -- Transporters at the blood-brain barrier.
Note:
Retrieved April 19, 2024, from https://hstalks.com/bs/761/.
,
Introduction -- Lecture outline -- The blood-brain-barrier -- Cellular architecture of the BBB -- Pericytes -- Molecular characteristics of the BBB -- Reference in vitro models of the BBB -- Cultured brain microvascular endothelial cells -- Phenotypic markers of brain endothelial cell -- BBB in vitro models -- Example of a co-culture system -- In vitro permeability assay -- Trans-endothelial electrical resistance (TEER) -- In vitro and in vivo drug permeability -- Drug permeability of various in vitro models -- Immortalization of rat brain endothelial cells -- Primary BMECs vs. immortalized cell lines -- A new model of human BBB -- Immortalization of human brain endothelial cells -- hCMEC/D3: a non-transformed phenotype (1) -- hCMEC/D3: a non-transformed phenotype (2) -- hCMEC/D3: expression of junction proteins (1) -- hCMEC/D3: expression of junction proteins (2) -- Permeability characteristics of hCMEC/D3 cells -- Efflux pupms: ABC transporters -- hCMEC/D3: functionality of ABC transporters -- The hCMEC/D3 cell line -- The hCMEC/D3 model under flow -- hCMEC/D3 in a DIV-BBB device -- Hyperosmolar opening of BBB in DIV models -- Radioactive sucrose permeability in DIV models -- hCMEC/D3 in a DIV-BBB device: conclusions -- Applications of in vitro models of the BBB -- BBB: physiological and pathophysiological roles -- In vitro BBB models and research axes -- Leukocyte infiltration across the BBB -- Response to cell adhesion and transmigration -- ICAM-1and PECAM-1 signaling -- Tight junctions, receptors and transporters -- Analysis of TJ proteomes in hCMEC/D3 cells -- Multiple reaction monitoring (MRM) -- Analysis by quantitative mass spectrometry -- Optimizing drug delivery to the brain -- BBB in vitro models: conclusions, perspectives -- Acknowledgments.
Language:
English
