UID:
almafu_9961427393202883
Umfang:
1 videorecording (54 min., 26 sec.) :
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sound, color
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005426
Inhalt:
Audio-visual presentation: Prions as infectious amyloids -- Yeast [PSI+] prion and Sup35p -- Molecular chaperone Hsp104 -- Guanidine hydrochloride as a prion propagation inhibitor -- Propagons -- Estimating propagon numbers using a stochastic model -- Generation of new propagons -- Propagation-defective mutants (PNM) -- Role of primary sequence information -- Yeast prion propagation: the unanswered questions.
Anmerkung:
Retrieved April 19, 2024, from https://hstalks.com/bs/962/.
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Introduction -- Prions -- Prions are infectious amyloids -- Amyloid fibres growth process -- Fragmentation of prion polymers -- Important questions concerning prions -- In vivo approach - prion propagation mechanism -- Yeast prion propagation and rapid cell division -- The PSI+ prion and its associated phenotype -- The prion protein Sup35p: eRF3 (1) -- Yeast prions are efficiently propagated -- Prion seeds and the 'propagon' -- The molecular chaperone Hsp104 discovery -- The molecular chaperone Hsp104 discovery -- Propagation of PSI+ prion and the role of Hsp104 -- Hsp104 activity Inhibition leads to propagons loss -- Guanidinium ion is an Hsp104-specific inhibitor -- GdnHCl eliminates [PSI+] prion from growing cells -- GdnHCl-induced [PSI+] curing and cell division (1) -- GdnHCl-induced [PSI+] curing and cell division (2) -- Calculating propagon numbers -- Proportion of propagons passed to daughter (1) -- Proportion of propagons passed to daughter (2) -- The advanced stochastic model -- Mechanism of new propagon generation -- The generation of new propagons -- Hsp104 elevated levels block [PSI+] propagation -- Isolation and genetic behaviour of PNM mutants -- PNM1 alleles of HSP104 show a defect in function -- The prion protein Sup35p: eRF3 (2) -- The prion protein Sup35p: eRF3 (3) -- PNM2-1 encodes a Sup35p defective form -- The effect of PNM2-1 mutation on propagation -- Sup35p primary sequence and [PSI+] propagation -- Other amino acid substitutions at Gly58 -- PNM2-1 mutation reduces PFD flexibility -- An overview of [PSI+] prion propagation -- Summary -- Acknowledgements -- References.
Sprache:
Englisch
