UID:
edocfu_9958083327902883
Format:
1 online resource (520 p.)
Edition:
2nd ed.
ISBN:
1-282-75560-9
,
9786612755606
,
0-12-375691-X
Content:
A must have for all Biotechnology Students and technicians. It walks readers through the minefield of laboratory calculations, helping to prevent the types of errors which could mean the loss of weeks of work.Key Features:* Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology* Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation* Recent applications of the procedures and computations in clinical, academic, industrial
Note:
Includes index.
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Front Cover; Calculations for Molecular Biology and Biotechnology; Copyright Page; Contents; 1 Scientific Notation and Metric Prefixes; Introduction; 1.1 Significant Digits; 1.1.1 Rounding Off Significant Digits in Calculations; 1.2 Exponents and Scientific Notation; 1.2.1 Expressing Numbers in Scientific Notation; 1.2.2 Converting Numbers from Scientific Notation to Decimal Notation; 1.2.3 Adding and Subtracting Numbers Written in Scientific Notation; 1.2.4 Multiplying and Dividing Numbers Written in Scientific Notation; 1.3 Metric Prefixes; 1.3.1 Conversion Factors and Canceling Terms
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Chapter Summary2 Solutions, Mixtures, and Media; Introduction; 2.1 Calculating Dilutions - A General Approach; 2.2 Concentrations by a Factor of X; 2.3 Preparing Percent Solutions; 2.4 Diluting Percent Solutions; 2.5 Moles and Molecular Weight - Definitions; 2.5.1 Molarity; 2.5.2 Preparing Molar Solutions in Water with Hydrated Compounds; 2.5.3 Diluting Molar Solutions; 2.5.4 Converting Molarity to Percent; 2.5.5 Converting Percent to Molarity; 2.6 Normality; 2.7 pH; 2.8 pK[sub(a)] and the Henderson-Hasselbalch Equation; Chapter Summary; 3 Cell Growth; 3.1 The Bacterial Growth Curve
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3.1.1 Sample Data3.2 Manipulating Cell Concentration; 3.3 Plotting OD[sub(550)] vs. Time on a Linear Graph; 3.4 Plotting the Logarithm of OD[sub(550)] vs. Time on a Linear Graph; 3.4.1 Logarithms; 3.4.2 Sample OD[sub(550)] Data Converted to Logarithm Values; 3.4.3 Plotting Logarithm OD[sub(550)] vs. Time; 3.5 Plotting the Logarithm of Cell Concentration vs. Time; 3.5.1 Determining Logarithm Values; 3.6 Calculating Generation Time; 3.6.1 Slope and the Growth Constant; 3.6.2 Generation Time; 3.7 Plotting Cell Growth Data on a Semilog Graph
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3.7.1 Plotting OD[sub(550)] vs. Time on a Semilog Graph3.7.2 Estimating Generation Time from a Semilog Plot of OD[sub(550)] vs. Time; 3.8 Plotting Cell Concentration vs. Time on a Semilog Graph; 3.9 Determining Generation Time Directly from a Semilog Plot of Cell Concentration vs. Time; 3.10 Plotting Cell Density vs. OD[sub(550)] on a Semilog Graph; 3.11 The Fluctuation Test; 3.11.1 Fluctuation Test Example; 3.11.2 Variance; 3.12 Measuring Mutation Rate; 3.12.1 The Poisson Distribution; 3.12.2 Calculating Mutation Rate Using the Poisson Distribution
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3.12.3 Using a Graphical Approach to Calculate Mutation Rate from Fluctuation Test Data3.12.4 Mutation Rate Determined by Plate Spreading; 3.13 Measuring Cell Concentration on a Hemocytometer; Chapter Summary; References; 4 Working with Bacteriophages; Introduction; 4.1 Multiplicity of Infection (moi); 4.2 Probabilities and Multiplicity of Infection (moi); 4.3 Measuring Phage Titer; 4.4 Diluting Bacteriophage; 4.5 Measuring Burst Size; Chapter Summary; 5 Nucleic Acid Quantification; 5.1 Quantification of Nucleic Acids by Ultraviolet (UV) Spectroscopy
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5.2 Determining the Concentration of Double-Stranded DNA (dsDNA)
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English
Additional Edition:
ISBN 0-12-375690-1
Language:
English
