UID:
edocfu_9960073438902883
Umfang:
1 online resource (203 p.)
ISBN:
1-281-75643-1
,
9786611756437
,
0-08-085881-3
Serie:
Laboratory techniques in biochemistry and molecular biology ; v. 12
Inhalt:
This volume is an extended account of a group of techniques that have seen their importance realized in almost all areas of biochemistry. The book provides detailed description of the properties and syntheses of the most useful photoactivatable reagents as well as comprehensive and critical descriptions of the major experiments that can be performed with photochemical reagents, affinity labelling, cross-linking of macromolecules and topographical analyses of membrane proteins.
Anmerkung:
Description based upon print version of record.
,
Front Cover; Photogenerated Reagents in Biochemistry and Molecular Biology; Copyright Page; Contents; Preface; List of abbreviations; Chapter 1. The utility of photoaffinity labeling and related methods; 1.1. The advantages of photogenerated reagents; 1.2. Some examples of photoaffinity labeling and related experiments; Chapter 2. Photogenerated reactive intermediates and their properties; 2.1. Carbenes; 2.2. Nitrenes; 2.3. Lifetimes of carbenes and nitrenes; 2.4. Free radicals and exited states; 2.5. Photosuicide inhibitors; Chapter 3. Reagents for photoaffinity labeling
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3.1. Requirements for a useful photoactivatable reagent3.2. Properties of aryl azides; 3.3. Properties of diazo compounds; 3.4. Properties of diazirines; 3.5. Synthesis of photoaffinity reagents; 3.6. Synthesis of reagents by chemical modification of macromolecules; 3.7. Radiolabeled reagents; Chapter 4. The photoaffinity labeling experiment; 4.1. Biological activity of the photoaffinity; 4.2. Sample preparation for photolysis; 4.3. Photolysis of the receptor-ligand complex; 4.4. Photoinactivation experiments; 4.5. Experiments with radioactive reagents; 4.6. The extent of labeling
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4.7. Control experiments4.8. Some miscellaneous methods; Chapter 5. Photochemical crosslinking reagents; 5.1. The advantages of photochemical reagents; 5.2. Reagents with two photoacitvatable groups; 5.3, The design of photochemical crosslinking reagents; 5.4. Experimental procedures; 5.5. Possible improvements of existing analytical methods; 5.6. Examples of photochemical crosslinking; 5.7. Improved crosslinking reagents; Chapter 6. Photoactivatable reagents for studying membrane topography; 6.1. Surface labeling reagents; 6.2. Hydrophobic reagents for membranes
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6.3. Amphipathic reagents for membranes6.4. Lipid derivatives; List of suppliers; References; Subject index
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English
Weitere Ausg.:
ISBN 0-444-80530-3
Sprache:
Englisch
