Format:
1 Online-Ressource (XXIV, 1110 Seiten)
ISBN:
9783527694464
,
9783527694471
,
9783527694440
Content:
Bioanalytics: Analytical Methods and Concepts in Biochemistry and Molecular Biology -- Table of Contents -- Preface -- Introduction: Bioanalytics - a Science in its Own Right -- Part I: Protein Analytics -- Chapter 1: Protein Purification -- 1.1 Properties of Proteins -- 1.2 Protein Localization and Purification Strategy -- 1.3 Homogenization and Cell Disruption -- 1.4 Precipitation -- 1.5 Centrifugation -- 1.5.1 Basic Principles -- 1.5.2 Centrifugation Techniques -- 1.6 Removal of Salts and Hydrophilic Contaminants -- 1.7 Concentration -- 1.8 Detergents and their Removal -- 1.8.1 Properties of Detergents -- 1.8.2 Removal of Detergents -- 1.9 Sample Preparation for Proteome Analysis -- Further Reading -- Chapter 2: Protein determination -- 2.1 Quantitative Determination by Staining Tests -- 2.1.1 Biuret Assay -- 2.1.2 Lowry Assay -- 2.1.3 Bicinchoninic Acid Assay (BCA Assay) -- 2.1.4 Bradford Assay -- 2.2 Spectroscopic Methods -- 2.2.1 Measurements in the UV Range -- 2.2.2 Fluorescence Method -- 2.3 Radioactive Labeling of Peptides and Proteins -- 2.3.1 Iodinations -- Further Reading -- Chapter 3: Enzyme Activity Testing -- 3.1 The Driving Force behind Chemical Reactions -- 3.2 Rate of Chemical Reactions -- 3.3 Catalysts -- 3.4 Enzymes as Catalysts -- 3.5 Rate of Enzyme-Controlled Reactions -- 3.6 Michaelis-Menten Theory -- 3.7 Determination of Km and Vmax -- 3.8 Inhibitors -- 3.8.1 Competitive Inhibitors -- 3.8.2 Non-competitive Inhibitors -- 3.9 Test System Set-up -- 3.9.1 Analysis of the Physiological Function -- 3.9.2 Selecting the Substrates -- 3.9.3 Detection System -- 3.9.4 Time Dependence -- 3.9.5 pH Value -- 3.9.6 Selecting the Buffer Substance and the Ionic Strength -- 3.9.7 Temperature -- 3.9.8 Substrate Concentration -- 3.9.9 Controls -- Further Reading -- Chapter 4: Microcalorimetry -- 4.1 Differential Scanning Calorimetry (DSC).
Note:
Includes index
Additional Edition:
ISBN 9783527339198
Additional Edition:
Erscheint auch als Druck-Ausgabe Bioanalytics Weinheim : Wiley-VCH Verlag GmbH & Co. KGaA, 2018 ISBN 9783527339198
Additional Edition:
ISBN 3527339191
Additional Edition:
Erscheint auch als Druck-Ausgabe Bioanalytics Weinheim : Wiley-VCH Verlag GmbH & Co. KGaA, 2018 ISBN 9783527339198
Additional Edition:
ISBN 3527339191
Language:
English
Subjects:
Biology
Keywords:
Biochemische Analyse
;
Biochemische Analyse
;
Molekularbiologie
;
Methode
Author information:
Lottspeich, Friedrich 1947-
