Umfang:
Online-Ressource (VIII, 101 p. 39 illus., 17 illus. in color, digital)
ISBN:
9783034805254
,
1283909146
,
9781283909143
Serie:
SpringerBriefs in Biochemistry and Molecular Biology
Inhalt:
Biological O2 sensing probes and measurement techniques were first introduced in the late 80s. In the last 3-5 years they have undergone major development that have made them available and affordable for a broad range of applications in various disciplines of the life and biomedical sciences. These new chemistries and technologies, which are significantly different from the majority of other fluorescence-based probes and detection techniques, have already demonstrated their high utility. This book will provide a systematic overview of the existing and emerging O2 sensing technologies in their different modifications, a practical guide to their rational selection and use, and examples of biological applications/case studies, including details on how to set up and conduct such experiments, troubleshoot and interpret the data.
Anmerkung:
Description based upon print version of record
,
2.2…Bioassays Performed with ecO2 Probes2.2.1 Analysis of Isolated Mitochondria; 2.2.2 Analysis of Mammalian Cell Respiration; 2.2.3 Analysis of Drug-Induced Mitochondrial Dysfunction by Parallel Measurement of OCR and Glycolytic Flux; 2.2.4 Multi-Parametric Assessment of 'Cell Energy Budget'; 2.2.5 Monitoring of Oxygenation and Cell Respiration in Microfluidic Biochips; 2.2.6 Analysis of Microbial Cell Cultures; 2.2.7 Respiration of Small Organisms and Model Animals; 2.2.8 Enzymatic Assays; 2.3…Bioassays Performed with icO2 Probes
,
2.3.1 Relationship Between Respiratory Activity, Sample Parameters and Oxygenation2.3.2 Cell Loading and Optical Measurements with icO2 Probes; 2.3.3 Probe Calibration; 2.3.4 Control of Oxygenation in Cultures of Adherent Cells; 2.3.5 Effects of Cell Metabolic Activity on icO2; 2.3.6 Multiple Treatments with Drugs and Monitoring of Transient Responses of Cells; 2.4…Instrument Requirements and Selection Criteria; 2.5…Conclusions; Acknowledgments; References; 3 O2 Imaging in Biological Specimens; Abstract; 3.1…Introduction; 3.2…Wide-Field Microsecond FLIM
,
3.3…Confocal Microsecond FLIM and Multi-Photon O2 Imaging3.4…Sample Preparation for Quantitative O2 Imaging; 3.4.1 Sample Temperature; 3.4.2 Properties of the Medium and pH; 3.4.3 Probe Photostability, Cyto- and Phototoxicity; 3.5…Loading of O2 Probes; 3.6…Settings for O2 Imaging on Camera-Based FLIM; 3.7…Acquisition of Images in Wide-Field FLIM Mode; 3.8…Calibration; 3.9…Factors Affecting the Quality of Wide-Field FLIM Images; 3.10…Examples of O2 Imaging Experiments; 3.10.1 Cultures of Adherent Cells; 3.10.1.1 Wide-Field FLIM; 3.10.1.2 Confocal TCSPC-FLIM
,
3.10.2 Multicellular Spheroids and Neurospheres3.10.2.1 Wide-Field Imaging of SK-N-AS Neurospheres; 3.10.2.2 Confocal TCSPC-FLIM of Primary Cortical Neurospheres; 3.10.2.3 Two-Photon Ratiometric O2 Imaging of Primary Cortical Neurospheres; 3.10.3 Microfluidic Systems and Cell-Based Biochips; 3.10.4 O2 Gradients in Cultures of C. elegans Worms; 3.11…Conclusions; Acknowledgments; References;
,
PhosphorescentOxygen-SensitiveProbes; Preface; Contents; 1 O2-Sensitive Probes Based on Phosphorescent Metalloporphyrins; Abstract; 1.1…Introduction; 1.2…Principles of Quenched-Phosphorescence Detection of O2; 1.3…Phosphorescent Metalloporphyrins and O2-Sensitive Materials on Their Basis; 1.4…Phosphorescent Probes for Sensing Cellular O2; 1.5…Detection Modalities; 1.6…Measurement Formats Used in Optical O2 Sensing; 1.7…Biological Applications of Optical O2 Sensing; Acknowledgments; References; 2 O2 Analysis on a Fluorescence Spectrometer or Plate Reader; Abstract; 2.1…Introduction
Weitere Ausg.:
ISBN 9783034805247
Weitere Ausg.:
Buchausg. u.d.T. Phosphorescent oxygen-sensitive probes Basel : Springer, 2012 ISBN 9783034805247
Weitere Ausg.:
ISBN 3034805241
Sprache:
Englisch
Fachgebiete:
Allgemeines
DOI:
10.1007/978-3-0348-0525-4
URL:
Volltext
(lizenzpflichtig)
