Format:
Online-Ressource (X, 336 p. 38 illus., 24 illus. in color, digital)
ISBN:
9783642324635
,
1283911825
,
9781283911825
Series Statement:
SpringerLink
Content:
Endocytosis is a fundamental cellular process by means of which cells internalize extracellular and plasma membrane cargos for recycling or degradation. It is important for the establishment and maintenance of cell polarity, subcellular signaling and uptake of nutrients into specialized cells, but also for plant cell interactions with pathogenic and symbiotic microbes. Endocytosis starts by vesicle formation at the plasma membrane and progresses through early and late endosomal compartments. In these endosomes cargo is sorted and it is either recycled back to the plasma membrane, or degraded in the lytic vacuole. This book presents an overview of our current knowledge of endocytosis in plants with a main focus on the key molecules undergoing and regulating endocytosis. It also provides up to date methodological approaches as well as principles of protein, structural lipid, sugar and microbe internalization in plant cells. The individual chapters describe clathrin-mediated and fluid-phase endocytosis, as well as flotillin-mediated endocytosis and internalization of microbes. The book was written for a broad spectrum of readers including students, teachers and researchers.
Note:
Description based upon print version of record
,
Endocytosis in Plants; Editor Biography; Preface; Contents; 1 Update on Methods and Techniques to Study Endocytosis in Plants; Abstract; 1…Introduction; 2…Chemical and Biochemical Methods; 2.1 Using Conventional Chemical Inhibitors to Study Endocytosis; 2.2 Chemical Genomics Identifies New Inhibitors of Endocytosis and Vesicular Trafficking; 2.3 Cell Fractionation and Isolation of Endosomes; 2.4 Isolation of Plasma Membrane Lipid Rafts; 2.5 Proteomic Approaches to Study Endocytosis; 3…Molecular Biological Methods
,
3.1 Cloning and Fluorescent Tagging of Endocytic Proteins for Visualization and Colocalization Studies3.2 Molecular Interactions Between Endocytic Proteins; 4…Genetic Methods; 5…Electrophysiological Methods; 6…Vital Fluorescent Markers for Endocytosis; 6.1 Fluorescently Labeled Endocytic Cargo; 6.2 Vital FM Styryl Dyes; 6.3 Filipin and di-4-ANEPPDHQ; 6.4 Labeled Ligands for Signalling Receptors; 7…Immunolocalization Methods for In Situ Localization of Endosomal Proteins; 8…Microscopic Methods; 8.1 Identification of New Endocytic Proteins by High-Throughput Microscopy Screen
,
8.2 Confocal Laser Scanning Microscopy8.3 Light Sheet-Based Fluorescence Microscopy; 8.4 Fluorescence Recovery After Photobleaching and Super-Resolution Microscopy; 8.5 Förster Resonance Energy Transfer; 8.6 Electron Microscopy and Electron Tomography; 9…Conclusions and Future Prospects; Acknowledgments; References; 2 Chemical Effectors of Plant Endocytosis and Endomembrane Trafficking; Abstract; 1…Endomembrane Trafficking in Plants Cells; 2…Chemicals Known to Perturb Endomembrane Trafficking; 3…Chemical Genomic Screens to Dissect Vacuole Transport and Biogenesis
,
4…Chemical Investigation of Development and Defense Responses5…Identifying New Inhibitors of Endosomes Involved in Polar Cell Growth; 6…Systematic Approach to Understand the Endomembrane Network; 7…Finding New Effectors and Pathways; References; 3 Cell Polarity and Endocytosis; Abstract; 1…Introduction; 2…Polarly Localized Proteins and their Roles; 3…Subcellular Trafficking in PIN Polarity; 4…PINOID Phosphorylation and ROP/RIC Interactions with Cytoskeleton: Common Mechanisms for PIN Polar Localization; 5…PIN Polarity Regulation in Response to Environmental Signals
,
6…A Mechanism for PIN Polar Maintenance7…Conclusions and Future Prospects; Acknowledgments; References; 4 Endocytosis and Vesicular Recycling in Root Hairs and Pollen Tubes; Abstract; 1…Introduction; 2…Structural and Functional Organization of Plant Tip-Growing Cells; 2.1 Tip Growth Requires Dynamic Cytoskeleton; 2.2 Vesicular Trafficking as an Integral Part of the Subcellular Membrane Flow; 3…Small GTPases: Master Determinants of Endocytosis and Vesicular Trafficking in Tip-Growing Cells; 3.1 Membrane Definition by Rab GTPases; 3.2 Signalling by Rop GTPases
,
4…Other Molecular Players Involved in Tip Growth
Additional Edition:
ISBN 9783642324628
Additional Edition:
Buchausg. u.d.T. Endocytosis in plants Berlin : Springer, 2012 ISBN 3642324622
Additional Edition:
ISBN 9783642324628
Language:
English
Subjects:
Biology
Keywords:
Pflanzenzelle
;
Endocytose
;
Aufsatzsammlung
DOI:
10.1007/978-3-642-32463-5
URL:
Volltext
(lizenzpflichtig)
