Format:
Online-Ressource (XI, 599 p. 231 illus., 84 illus. in color, online resource)
ISBN:
9783642544521
Series Statement:
RNA Technologies
Content:
This volume contains 29 engrossing chapters contributed by worldwide, leading research groups in the field of chemical biology. Topics include pre-biology; the establishment of the genetic code; isomerization of RNA; damage of nucleobases in RNA; the dynamic structure of nucleic acids and their analogs in DNA replication, extra- and intra-cellular transport; molecular crowding by the use of ionic liquids; new technologies enabling the modification of gene expression via editing of therapeutic genes; the use of riboswitches; the modification of mRNA cap regions; new approaches to detect appropriately modified RNAs with EPR spectroscopy and the use of parallel and high-throughput techniques for the analysis of the structure and new functions of nucleic acids. This volume discusses how chemistry can add new frontiers to the field of nucleic acids in molecular medicine, biotechnology and nanotechnology and is not only an invaluable source of information to chemists, biochemists and life scientists but will also stimulate future research.
Note:
Description based upon print version of record
,
Preface; References; Contents; RNA as Major Components in Chemical Evolvable Systems; 1 Introduction: Synthetic Living Cells from an Experimental Bottom-Up Approach; 1.1 What I Cannot Create, I Do Not Understand; 1.2 Hierarchical Reduction, Metaphors, Prudent Compromises and Dogma 13; 2 First Steps: Preparations for the Real Thing; 2.1 Translate to Replicate, Rather than an RNA World; 2.2 How to Compartment RNA?; 2.3 A Hydrophobic Peptide Anchors RNA to Giant Lipidic Vesicles; 2.3.1 A Non-dynamic Model System; 2.3.2 Lipid Vesicles as Hosts; 2.3.3 Peptidyl RNA: An Informational Amphiphile
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2.3.4 Anchoring, Partitioning and (Im)Permeability2.3.5 Results; Confocal Fluorescence Microscopy on Naturally Swelled GVs; Confocal Fluorescence Microscopy on GVs Incubated with Peptidyl RNA; Light Scattering, Turbidity and Permeability Assays on LUVs; 2.3.6 Data Analysis and Discussion; 2.3.7 Quod Nulla Capra Abligurrit !; 2.4 How to Generate Plausibly Prebiotic Lipophilic ``Random´´ Peptide Libraries?; 2.4.1 Natural and Designed Cell Penetrating Peptides and Pore-Forming Proteins; 2.4.2 Potentially Functional ``Random´´ Co-oligopeptides; 3 Perspectives: Evolvable Systems Chemistry
,
3.1 Lipids and Glycans3.2 Peptidyl RNA Esters; 3.3 Why RNA?; References; How the Early Genetic Code Was Established?: Inference from the Analysis of Extant Animal Mitochondrial Decoding Systems; 1 Introduction; 2 Comparison of Genetic Apparatus in Mitochondrial and Non-mitochondrial Decoding Systems; 3 Characteristics of Mitochondrial Genetic Code Systems; 4 The Codon-Anticodon Relationship in Animal Mitochondria; 5 How Was the Mitochondrial Decoding System Generated?; 6 The Early Genetic Code System; 7 From the Early Decoding System to the Universal Decoding System
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8 Concluding Remarks and PerspectiveReferences; Isomerization of RNA Phosphodiester Linkages; 1 Introduction; 2 Isomerization Under Acidic Conditions via a Neutral Oxyphosphorane Intermediate; 3 pH-Independent Isomerization via a Monoanionic Phosphorane Intermediate; 4 Isomerization via Dianionic Phosphorane: Effect of Intramolecular Hydrogen Bonding; 5 Buffer-Catalyzed Isomerization; 6 Metal Ion-Promoted Isomerization; 7 Effect of Thio Substitution and Alkylation of a Non-bridging Oxygen on Isomerization; 8 Epilogue; References
,
Effects of Ionic Liquid and Liposomes on the Structure, Stability, and Function of Nucleic Acids1 Introduction; 2 Nucleic Acids with and on Liposomes; 3 Nucleic Acids in a Hydrated Ionic Liquid; 4 New Sensing System for DNA Sequences; 5 Perspectives; References; Oxidative Damage on RNA Nucleobases; 1 Introduction; 2 Definition of Reactive Oxygen and Nitrogen Species and Their Properties; 3 Reactivity of ROS and RNS Versus Nucleic Acids; 4 Oxidative Lesions in RNA; 4.1 Base-Pairing Properties of Lesion Containing Duplexes; 4.2 Mutagenicity of Oxidatively Damaged Nucleobases
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4.2.1 5-Hydroxypyrimidines
Additional Edition:
ISBN 9783642544514
Additional Edition:
Erscheint auch als Druck-Ausgabe Chemical biology of nucleic acids Berlin : Springer, 2014 ISBN 9783642544514
Additional Edition:
ISBN 3642544517
Language:
English
Subjects:
Biology
Keywords:
Nucleinsäuren
;
Molekularbiologie
;
Molekulare Medizin
;
Nucleinsäuren
;
Biochemie
;
Nucleinsäuren
;
Klinische Medizin
;
Aufsatzsammlung
DOI:
10.1007/978-3-642-54452-1
