Umfang:
1 Online-Ressource (xii, 226 Seiten)
,
Illustrationen, Diagramme
Ausgabe:
First edition
ISBN:
9780191864711
Serie:
Oxford graduate texts
Inhalt:
This book presents a comprehensive overview of the foundations of single-molecule studies, based on manipulation of the molecules and observation of these with fluorescent probes. It first discusses the forces present at the single-molecule scale, the methods to manipulate them, and their pros and cons. It goes on to present an introduction to single-molecule fluorescent studies based on a quantum description of absorption and emission of radiation due to Einstein. Various considerations in the study of single molecules are introduced (including signal to noise, non-radiative decay, triplet states, etc.) and some novel super-resolution methods are sketched. The elastic and dynamic properties of polymers, their relation to experiments on DNA and RNA, and the structural transitions observed in those molecules upon stretching, twisting, and unzipping are presented. The use of these single-molecule approaches for the investigation of DNA–protein interactions is highlighted via the study of DNA and RNA polymerases, helicases, and topoisomerases. Beyond the confirmation of expected mechanisms (e.g., the relaxation of DNA torsion by topoisomerases in quantized steps) and the discovery of unexpected ones (e.g., strand-switching by helicases, DNA scrunching by RNA polymerases, and chiral discrimination by bacterial topoII), these approaches have also fostered novel (third generation) sequencing technologies.
Anmerkung:
Literaturverzeichnis: Seite 201-221
Weitere Ausg.:
ISBN 9780198530923
Weitere Ausg.:
Erscheint auch als Druck-Ausgabe Bensimon, David, 1954 - Single molecule studies of nucleic acids and their associated proteins Oxford : Oxford University Press, 2019 ISBN 9780198530923
Sprache:
Englisch
Fachgebiete:
Chemie/Pharmazie
Schlagwort(e):
Nucleinsäuren
;
Proteine
;
Fluoreszenzspektroskopie
DOI:
10.1093/oso/9780198530923.001.0001
