Format:
1 Online-Ressource (vii, 87, VI Seiten, 5610 KB)
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Illustrationen, Diagramme
Content:
An important goal in biotechnology and (bio-) medical research is the isolation of single cells from a heterogeneous cell population. These specialised cells are of great interest for bioproduction, diagnostics, drug development, (cancer) therapy and research. To tackle emerging questions, an ever finer differentiation between target cells and non-target cells is required. This precise differentiation is a challenge for a growing number of available methods. Since the physiological properties of the cells are closely linked to their morphology, it is beneficial to include their appearance in the sorting decision. For established methods, this represents a non addressable parameter, requiring new methods for the identification and isolation of target cells. Consequently, a variety of new flow-based methods have been developed and presented in recent years utilising 2D imaging data to identify target cells within a sample. As these methods aim for high throughput, the devices developed typically require highly complex fluid handling techniques, making them expensive while offering limited image quality. [...]
Note:
Dissertation Universität Potsdam 2023
Additional Edition:
Erscheint auch als Druck-Ausgabe Gerling, Marten Tobias A microfluidic system for high-precision image-based live cell sorting using dielectrophoretic forces Potsdam, 2022
Language:
English
Keywords:
Hochschulschrift
DOI:
10.25932/publishup-58742
URN:
urn:nbn:de:kobv:517-opus4-587421
Author information:
Bier, Frank Fabian 1959-
Author information:
Beta, Carsten 1974-