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Format: Online-Ressource (XXIX, 600 S.)

Edition: Online-Ausg. 2010 Electronic reproduction

ISBN: 9780121820817

Series Statement: RNA processing / ed. by James E. Dahlberg ... Pt. A

Content: Many methods have been developed or adapted for the study of RNA processing, which plays a key role in the expression of genetic information. Volumes 180 and 181 of Methods in Enzymology, RNA Processing, Parts A and B, represent the first comprehensive collation of protocols used for the preparation of substrates, for the preparation of extracts and enzymes, and for the analysis of products in RNA processing. At least half of the chapters present techniques that are directly applicable to other areas of nucleic acid biochemistry and cell biology

Note: Includes bibliographical references , Use copy Restrictions unspecified star MiAaHDL , A guide for probing native small nuclear RNA and ribonucleoprotein structures , Isolation of RNA , Phylogenetic comparative analysis of RNA secondary structure , Mapping the genetic organization of RNA by electron microscopy , Computer prediction of RNA structure , RNA pseudoknot: Structure, detection, and prediction , absorbance melting curves of RNA , Nuclease digestion: A method for mapping introns , Characterization of RNA molecules by S1 nuclease analysis , Primer extension analysis of RNA , Modification interference analysis of reactions using RNA substrates , Photoaffinity cross-linking methods for studying RNA-protein interactions , Ultraviolet-induced cross-linking of rna to proteins in vivo , Analysis of ultraviolet-induced RNA-RNA cross-links: A means for probing RNA structure-function relationships , analysis of splicing complexes and small nuclear ribonucleoprotein particles by native gel electrophoresis , Determination of RNA-protein and RNA-ribonucleoprotein interactions by nuclease probing , Immunoprecipitation of ribonucleoproteins using autoantibodies , Electron microscopy of ribonucleoprotein complexes on nascent RNA using miller chromatin spreading method , Genetic Methods for identification and characterization of RNA-RNA and RNA-protein interactions , Compilation of small nuclear RNA sequences , Sequences and classification of group I and group II introns , Compilation of self-cleaving sequences from plant virus satellite RNAs and other sources , Purification of RNA molecules gel techniques , Purifying RNA by column chromatography , Synthesis of long, capped transcripts in Vitro by SP6 and T7 RNA polymerases , Synthesis of small RNAs using T7 RNA polymerase , In vitro synthesis of end-mature, intron-containing transfer RNAs , Preparation of precursors to mRNA from mammalian cell nuclei , Pulse labeling of heterogeneous nuclear RNA in isolated nuclei , Isolation and characterization of yeast ribosomal RNA precursors and preribosomes , Preparation of yeast transfer RNA precursors in Vivo , Dideoxy sequencing of RNA using reverse transcriptase , RNA fingerprinting , Enzymatic RNA sequencing , Characterization of cap structures , Isolation and characterization of branched oligonucleotides from RNA , Enzymatic approaches to probing of RNA secondary and tertiary structure , Electronic reproduction

Additional Edition: Erscheint auch als Druck-Ausgabe RNA processing ; Pt. A: General methods San Diego, Calif. [u.a.] : Academic Press, 1989 ISBN 0121820815

Language: English

URL: Volltext

URL: Volltext

URL: Volltext

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