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Format: Online-Ressource (XXXIII, 746 S.)

Edition: Online-Ausg. 2010 Electronic reproduction

ISBN: 9780121820855

Series Statement: Methods in enzymology 184

Content: The avidin-biotin complex has been used for isolation (affinity chromatography), localization (affinity cytochemistry, cell cytometry, and blotting technology), and diagnostics (immunoassay, histopathology, and gene probes). Recently, usage of the system has been extended to include other areas. This volume covers these new applications and methodologies including hybridoma technology, bioaffinity sensors, affinity targeting, and drug delivery, as well as cross-linking, immobilization, and fusogenic studies

Note: Includes bibliographical references , Includes indexes , Use copy Restrictions unspecified star MiAaHDL , Biotinylated protein A in immunoassay , Double fluorescence immunolabeling studies , Reflections , Literaturangaben , Slide immunoenzymatic assay for human immunoglobulin E , Quantification of carcinoembryonic antigen in serum and analysis of epitope specificities of monoclonal antibodies , Selective precipitation of biotin-labeled antigens or monoclonal antibodies by avidin for determining epitope specificities and affinities in solution-phase assays , Immunoassays for diagnosis of infectious diseases , Enzyme-linked immunosorbent assay , Rapid detection of herpes simplex virus , Sensitive immunoassay for human immunodeficiency viral core proteins , Gene probes , Biotinylated nucleotides for labeling and detecting DNA , Biotinylated psoralen derivative for labeling nucleic acid hybridization probes , Chemically cleavable biotin-labeled nucleotide analogs , Preparation and uses of photobiotin , Modification of cytosine residues on DNA , Direct incorporation of biotin into DNA , Colorimetric-Detected DNA Sequencing , Composite applications , Third component of human complement: C3 , Purification and characterization of membrane proteins , Facilitated cell fusion for hybridoma production , Complex neoglycoproteins , Probing [beta]-adrenergic receptors , Probing adenosine receptors using biotinylated purine derivatives , Detection of cell surface components using fluorescent microspheres , Immunohistochemistry , Immunocytochemical detection of human T- and B-cell antigens , Ultrastructural localization of retinal photoreceptor proteins , Visualization of intracellular trafficking of proteins , Localization of lectin receptors , Localization of gonadotropin-releasing hormone receptors , Localization of ganglioside GM1 with biotinylated choleragen , Visualization of adenosinetriphosphatase site of myosin using photoreactive adenosine 5'-diphosphate analog , Analysis of proteins and glycoproteins on blots , Direct labeling of blotted glycoconjugates , Identification of leukocyte surface proteins , Staining of proteins on nitrocellulose replicas , Streptavidin-Enzyme complexes in detection of antigens on western blots , Luminescent detection of immunodot and western blots , Identification of calmodulin-binding proteins , Avidin-biotin mediated immunoassays: Overview , Avidin-biotin system in enzyme immunoassays , Two-site and competitive chemiluminescent immunoassays , Introduction to avidin-biotin technology , Applications of avidin-biotin technology: Literature survey , Biotin-binding proteins: Overview and prospects , Avidin and streptavidin , Nonglycosylated avidin , Cloning and expression of avidin in Escherichia coli , Isolation and properties of streptavidin , Crystal forms of avidin , Nonavidin biotin-binding proteins , Biotinidase , Monoclonal antibody to biotin , Biotin-containing reagents , Protein biotinylation , Enzymatic C-terminal biotinylation of proteins , Antibodies biotinylated via sugar moieties , 2-Iminobiotin-containing reagent and affinity columns , Avidin- and streptavidin-containing probes , Phycobiliprotein--avidin and phycobiliprotein--biotin conjugates , Monovalent avidin affinity columns , Prevention of nonspecific binding of avidin , Enzymatic and radioactive assays for biotin, avidin, and streptavidin , Colorimetric enzyme assays for avidin and biotin , Sequential solid-phase assay for biotin based on 125I-labeled avidin , Fluorometric assay for avidin--biotin interaction , Isolation of biologically active compounds: A universal approach , Isolation and characterization of hormone receptors , Biotinylation of peptide hormones: Structural analysis and application to flow cytometry , Purification of gonadotropin-releasing hormone receptors , Biotinylestradiol for purification of estrogen receptor , One-step immunoaffinity purification of transferrin , Lectin-mediated isolation of cell surface glycoproteins , Immunoselective cell separation , Isolation and analysis of splicing complexes , Z-DNA affinity chromatography , Affinity cytochemistry , Electronic reproduction

Additional Edition: Erscheint auch als Druck-Ausgabe Avidin-biotin technology San Diego, Calif. [u.a.] : Academic Press, 1990 ISBN 0121820858

Language: English

Subjects: Biology

URL: Volltext

URL: Volltext

URL: Volltext