Format:
Online-Ressource
,
v.: digital
Edition:
1
Edition:
Online-Ausg. Springer eBook Collection. Biomedical and Life Sciences Electronic reproduction; Available via World Wide Web
ISBN:
9781441912602
Series Statement:
Reviews in Fluorescence 2008 2008
Content:
This is the fifth volume in the Reviews in Fluorescence series. To date, four volumes have been both published and well received by the scientific community. Several book reviews in the last few years have also favourably remarked on the series. In this 5th volume we continue the tradition of publishing leading edge and timely articles from authors around the world. We thank the authors for their timely and exciting contributions. We hope you will find this volume as useful as past volumes, which promises to be just as diverse with regard to fluorescence-based content
Note:
Includes index
,
Preface; Contents; Contributors; Fluorescence Anisotropy to Study the Preferential Orientation of Fluorophores in Ordered Bi-Dimensional Systems: Rhodamine 6G/Laponite Layered Films; 1 Introduction; 2 Fluorescence Anisotropy in Ordered Bi-Dimensional Systems; 3 Dye/Clay Systems: Film Characterization; 4 Dye Orientation in Ordered Clay Films. A Fluorescence Anisotropy Study; 5 Conclusions; References; Room Temperature Tryptophan Phosphorescence of Proteins in the Composition of Biological Membranes and Solutions; 1 Introduction
,
2 Room Temperature Tryptophan Phosphorescence of Proteins of Isolated Human Erythrocyte Membranes3 Room Temperature Tryptophan Phosphorescence of Plant Lectins in Solution; 3.1 Concanavalin A (Con A); 3.2 Phytohemagglutinin-L (PHA-L); 3.3 Wheat Germ Agglutinin (WGA); 3.4 Peanut Agglutinin (PNA); 3.5 Pisum sativum Agglutinin (PSA); 3.6 Sambucus nigra Agglutinin (SNA-I); 3.7 Laburnum anagyroides Lectin (LAL); 3.8 Solanum tuberosum Agglutinin (STA); References; Rational Design of FRET-Based Sensor Proteins; 1 Introduction
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2 Factors That Affect the Ratiometric Change in FRET-Based Sensor Proteins3 Quantitative Understanding of Energy Transfer by Modeling the Conformational Behavior of Flexible Linkers; 4 Quantitative Understanding of the Effect of Flexible Peptide Linkers on Effective Concentration; 5 Chelating Fluorescent Protein Chimeras as Efficient Zn(II) Sensor Proteins; 6 Taking Advantage of Stickiness: FRET Sensor Proteins Based on Conformational Switching; 7 Conclusion and Outlook; References; Fluorescence Imaging of Calcium Loading and Mitochondrial Depolarization in Cancer Cells Exposed to Heat Stress
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1 Introduction2 Fluorescence Imaging of Mitochondrial Calcium Loading; 2.1 The Role of Ca 2+ Inside Cells; 2.1.1 Fluorescent Ca 2+ Indicators; 2.2 Materials and Methods; 3 Results; 3.1 Cell Viability; 3.2 Visual Evaluation of Fluorescent Ca 2+ Indicators; 3.3 Fluorescence Imaging of Mitochondrial Transmembrane Potential; 4 Discussion; 5 Conclusions; References; Energy Transfer in Silica Nanoparticles: An Essential Toolfor the Amplification of the Fluorescence Signal; 1 Introduction; 2 The Principles of Energy Transfer Processes; 3 The Power of Energy Transfer Processes
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4 The Synthesis of Luminescent Silica Nanoparticles5 Photophysical Properties of Luminescent Silica Nanoparticles; 6 Conclusions; References; Spectroscopic Characterization of PlasmaChemically Functionalized and Fluorophore-Labeled Polymer Surfaces; 1 Introduction; 2 Surface Modification; 2.1 Surface Functionalization of Polypropylene; 2.2 Determination of Surface Functionalities with XPS; 2.3 Fluorophore Labeling of PP Surfaces; 3 Characterization of PP Films with Commonly Used Surface-Sensitive Techniques
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4 Fluorescence-Based Characterization of Labeled PP Surfaces Pitfalls and Troubleshooting
,
Electronic reproduction; Available via World Wide Web
Additional Edition:
ISBN 9781441908285
Language:
English
DOI:
10.1007/978-1-4419-1260-2
URL:
Volltext
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