Format:
Online-Ressource (XV, 483 S., [16] Bl.)
Edition:
Online-Ausg. Electronic reproduction; Available via World Wide Web
ISBN:
9780470952511
,
1118523180
,
1118523067
,
1118523202
,
1118523172
,
9781118523186
,
9781118523063
,
9781118523209
,
9781118523179
Series Statement:
Wiley Series on Surface and Interfacial Chemistry
Content:
"Proteins in solution and at interfaces are increasingly used in exciting new applications, from biomimetic materials to nanoparticle patterning. This book surveys the state-of-the-art in the field, providing scientists in diverse areas with a comprehensive and modern analysis of the techniques used in protein characterization, as well as insight into the most important applications. Topics include protein and protein aggregate structure; computational and experimental techniques for the study of protein structure, aggregation, and adsorption; proteins in non-standard conditions; and biotechnological applications"--
Content:
"This book offers a comprehensive and modern analysis of the techniques used in protein characterization, as well as an approach to the most demanding applications today and the most promising for the future"--
Content:
"This book offers a comprehensive and modern analysis of the techniques used in protein characterization, as well as an approach to the most demanding applications today and the most promising for the future"--
Content:
"Proteins in solution and at interfaces are increasingly used in exciting new applications, from biomimetic materials to nanoparticle patterning. This book surveys the state-of-the-art in the field, providing scientists in diverse areas with a comprehensive and modern analysis of the techniques used in protein characterization, as well as insight into the most important applications. Topics include protein and protein aggregate structure; computational and experimental techniques for the study of protein structure, aggregation, and adsorption; proteins in non-standard conditions; and biotechnological applications"--
Note:
Includes bibliographical references and index
,
PROTEINS IN SOLUTION AND AT INTERFACES; CONTENTS; PREFACE; CONTRIBUTORS; PART I; 1 X-RAY CRYSTALLOGRAPHY OF BIOLOGICAL MACROMOLECULES: FUNDAMENTALS AND APPLICATIONS; 1.1 INTRODUCTION; 1.2 FUNDAMENTALS OF X-RAY DIFFRACTION; 1.2.1 X-Ray Radiation and Interaction with Matter; 1.2.2 Crystals and Symmetry; 1.2.3 Diffraction by Crystals; 1.2.4 Real and Reciprocal Space; 1.2.5 Structure Factors; 1.2.6 Fourier Synthesis and Transform; 1.2.7 The Phase Problem; 1.3 THE STRUCTURE DETERMINATION PROCESS; 1.3.1 Sample Production and Conditioning; 1.3.2 Crystallization; 1.3.3 Data Collection and Processing
,
1.3.4 Structure Determination1.3.5 Electron Density Map Interpretation: Model Construction; 1.3.6 Model Refinement; 1.3.7 Validation; 1.4 STRUCTURAL ANALYSIS AND BIOLOGICAL IMPLICATIONS; 1.4.1 Structural Analysis; 1.4.2 Biological Implications; 1.5 FUTURE PROSPECTS; ACKNOWLEDGMENTS; REFERENCES; 2 NUCLEAR MAGNETIC RESONANCE METHODS FOR STUDYING SOLUBLE, FIBROUS, AND MEMBRANE-EMBEDDED PROTEINS; 2.1 INTRODUCTION AND BACKGROUND; 2.1.1 Nuclear Angular Momentum; 2.1.2 Chemical Shifts; 2.1.3 Nuclear Spin Interactions; 2.1.4 Relaxation; 2.1.5 Isotopic Labeling; 2.1.6 Samples; 2.2 STRUCTURAL DATA
,
2.2.1 Resonance Assignment2.2.2 Distance Measurements; 2.2.3 Angular Information; 2.2.4 Residual Dipolar Couplings; 2.2.5 Use of Paramagnetic Agents; 2.2.6 Oriented Samples; 2.2.7 Structure Calculations; 2.3 DYNAMICS; 2.3.1 Fast (ps-ns) Motions; 2.3.2 Slow (μs-ms) Motions; 2.3.3 Motions in Solids; 2.4 INTERMOLECULAR INTERACTIONS; 2.4.1 Identification of Interaction Surfaces; 2.4.2 Structural Restraints; 2.5 DISCUSSION; 2.5.1 Large Protein Systems; 2.5.2 Advantages and Disadvantages of Solution and Solid-State MAS NMR; 2.5.3 Complementary Techniques; 2.6 CONCLUSION; REFERENCES
,
3 SMALL-ANGLE X-RAY SCATTERING APPLIED TO PROTEINS IN SOLUTION3.1 INTRODUCTION; 3.2 SAXS THEORY; 3.2.1 General Equations; 3.2.2 Isotropy; 3.2.3 Homogeneous Scattering Particles (Two-Phase Model); 3.2.4 Model-Independent Shape Analysis: Kratky's Representation, Guinier's Law, and Porod's Invariant; 3.2.5 Methods to Calculate P(q); 3.2.6 Protein-Protein Interaction: S(q) Evaluation; 3.3 EXAMPLES; 3.3.1 Rg, p(r), Kratky's Representation and Shape Reconstruction: Analysis of Protein TcOYE; 3.3.2 Guinier's Analysis and Multipole Expansion Shape Reconstruction: The Replication Factor C Case
,
3.3.3 Partial Folded and Unfolded Protein: Analysis of the Protein Bovine Serum Albumin, BSA, Under the Influence of Denaturing Agents3.4 PROTEIN INTERACTION: BSA CASE; 3.5 PROTEIN AGGREGATION; 3.6 CONCLUSION; ACKNOWLEDGMENTS; REFERENCES; 4 ANALYZING THE SOLUTION STATE OF PROTEIN STRUCTURE, INTERACTIONS, AND LIGANDS BY SPECTROSCOPIC METHODS; 4.1 INTRODUCTION; 4.1.1 Overview; 4.1.2 Protein Secondary Structures and Motifs; 4.1.3 Structure Determination: Spectroscopic Methods; 4.2 ULTRAVIOLET-VISIBLE ABSORPTION SPECTROSCOPY; 4.2.1 Background
,
4.2.2 Lambert-Beer Law: Determination of Protein Concentration
,
Electronic reproduction; Available via World Wide Web
Additional Edition:
ISBN 9781118523179*ePub
Additional Edition:
ISBN 9781118523186*Adobe PDF
Additional Edition:
ISBN 9781118523209* MobiPocket
Additional Edition:
ISBN 9781118523063
Additional Edition:
Erscheint auch als Druck-Ausgabe Proteins in solution and at interfaces Hoboken, NJ : Wiley, 2013 ISBN 9780470952511
Language:
English
Subjects:
Chemistry/Pharmacy
,
Biology
Keywords:
Proteine
;
Biotechnologie
DOI:
10.1002/9781118523063
