Umfang:
Online-Ressource (XV, 356 S.)
,
Ill., graph. Darst.
Ausgabe:
Online-Ausg.
ISBN:
9780128015117
Serie:
Methods in enzymology 555
Inhalt:
These new volumes of Methods in Enzymology (554 and 555) on Hydrogen Sulfide Signaling continue the legacy established by previous volumes on another gasotransmitter, nitric oxide (Methods in Enzymology volumes 359, 396, 440, and 441), with quality chapters authored by leaders in the field of hydrogen sulfide research. These volumes of Methods in Enzymology were designed as a compendium for hydrogen sulfide detection methods, the pharmacological activity of hydrogen sulfide donors, the redox biochemistry of hydrogen sulfide and its metabolism in mammalian tissues, the mechanisms inherent in hy
Anmerkung:
Description based upon print version of record
,
Front Cover; Hydrogen Sulfide in Redox Biology, Part B; Copyright; Contents; Contributors; Preface; Section I: The Redox Biochemistry of Hydrogen Sulfide; Chapter 1: Investigating the Role of H2S in 4-HNE Scavenging; 1. Introduction; 2. Experimental Compounds and Considerations; 2.1. H2S generation; 2.2. Preparation of 4-HNE solutions; 2.3. Preparation of 4-HNA solutions; 2.4. Reaction of 4-HNE with H2S; 2.5. Approaches to monitor the effect of H2S on protein modification by 4-HNE; 2.5.1. Electrophoretic mobility shift; 2.5.2. Immunoblotting
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2.6. Approaches to monitor the effect of H2S on cytotoxicity of 4-HNE2.7. Detection of 4-HNE-modified cellular proteins; 3. Conclusions and Perspectives; Acknowledgment; References; Chapter 2: Inhalation Exposure Model of Hydrogen Sulfide (H2S)-Induced Hypometabolism in the Male Sprague-Dawley Rat; 1. Introduction; 2. Exposure Protocol for H2S-Induced Hypometabolism in Rats; 2.1. Special gases and equipment; 2.2. Animals; 2.3. H2S exposure protocol; 2.4. Physiological measurements; 2.5. Lung histopathology assessment
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2.6. Tissue and blood collection after H2S exposure for experimental measurements2.7. Comments on measuring H2S and H2S-derived metabolites in blood and tissues from H2S-exposed rats; 3. Other Considerations for H2S Exposure Studies; 4. Summary; Acknowledgments; References; Section II: Mechanisms of H2S Cell Signaling and Transcriptional Pathways; Chapter 3: Use of the ""Tag-Switch"" Method for the Detection of Protein S-Sulfhydration; 1. Introduction; 2. The Design of ""Tag-Switch"" Method; 3. Chemistry Validation Using Small-Molecule Substrates; 3.1. Materials
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3.2. Model reactions of MSBT with thiol and persulfide substrates3.3. Model reaction of cyanoacetate with R-S-S-BT; 4. ""Tag-Switch"" Assay on Bovine Serum Albumin and GAPDH as Model Proteins; 4.1. Materials; 4.2. Preparation of BSA-SOH, BSA-SSG, and BSA-SSH; 4.3. ""Tag-switch"" labeling of model proteins for MS and dot blot; 4.4. Studying the mechanism of protein S-sulfhydration by ""tag-switch"" assay; 5. ""Tag-Switch"" Assay for the Detection on Intracellular Protein Persulfides; 5.1. Materials; 5.2. Tag-switch labeling of Jurkat cell extracts
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5.3. Tag-switch labeling during immunoprecipitation6. ""Tag-Switch"" Assay for the Detection of Intracellular S-Sulfhydration by Fluorescence Microscopy; 6.1. Materials; 6.2. Protocol; 6.3. Colocalization of intracellular S-sulfhydration with different organelles; 7. Conclusions; Acknowledgments; References; Chapter 4: Real-Time Assays for Monitoring the Influence of Sulfide and Sulfane Sulfur Species on Protein Thiol Redox States; 1. Introduction; 2. PTEN Activity Assay; 2.1. Principle; 2.2. Reagents and equipment; 2.3. Procedure; 2.4. Comments; 2.5. Expected results; 3. roGFP2 Redox Assay
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3.1. Principle
Weitere Ausg.:
ISBN 9780128016220
Weitere Ausg.:
Erscheint auch als Druck-Ausgabe Hydrogen Sulfide in Redox Biology Part B
Sprache:
Englisch
Schlagwort(e):
Schwefelwasserstoff
;
Redoxreaktion
;
Aufsatzsammlung
