Format:
Online-Ressource (1 v)
Edition:
Online-Ausg.
ISBN:
9780123705013
,
6611370900
,
9786611370909
Content:
Bioconjugate Techniques, 2e provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. *A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab, allowing for fast retrieval of tried and tested techniques for synthesizing bioconjugates. *Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates. *Includes more than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates, giving researchers a better understanding of how the commercially available reagents facilitate the crosslinking of peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. *Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticles Silane coupling agents Dendrimers and dendrons Chemoselective ligation Quantum dots Lanthanide chelates Cyanine dyes Discrete PEG compounds Buckyballs, fullerenes, and carbon nanotubes Mass tags and isotope tags Bioconjugation in the study of protein interactions
Content:
Bioconjugate Techniques, 2e provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. *A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab, allowing for fast retrieval of tried and tested techniques for synthesizing bioconjugates. *Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates. *Includes more than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates, giving researchers a better understanding of how the commercially available reagents facilitate the crosslinking of peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. *Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticles Silane coupling agents Dendrimers and dendrons Chemoselective ligation Quantum dots Lanthanide chelates Cyanine dyes Discrete PEG compounds Buckyballs, fullerenes, and carbon nanotubes Mass tags and isotope tags Bioconjugation in the study of protein interactions
Note:
Includes bibliographical references (p. 1041-1132) and index
,
Functional targetsThe chemistry of reactive groups -- Zero-length cross-linkers -- Homobifunctional cross-linkers -- Heterobifunctional cross-linkers -- Trifunctional cross-linkers -- Dendrimers and dendrons -- Cleavable reagent systems -- Fluorescent probes -- Bifunctional chelating agents and radioimmunoconjugates -- Biotinylation reagents -- Iodination reagents -- Silane coupling agents -- Microparticles and nanoparticles -- Buckyballs, fullerenes and carbon nanotubes -- Mass tags and isotope tags -- Chemoselective ligation: bioorthogonal reagents -- Discrete PEG reagents -- Preparation of hapten-carrier immunogen conjugates -- Antibody modification and conjugation -- Immunotoxin conjugation techniques -- Preparation of liposome conjugates and derivatives -- Avidin-biotin systems -- Preparation of colloidal gold-labeled proteins -- Modification with synthetic polymers -- Enzyme modification and conjugation -- Nucleic acid and oligonucleotide modification and conjugation -- Bioconjugation in the study of protein interactions.
Additional Edition:
ISBN 0123705010
Additional Edition:
ISBN 6611370900
Additional Edition:
ISBN 9780123705013
Additional Edition:
ISBN 9786611370909
Language:
English
Subjects:
Biology
Keywords:
Chemische Reaktion
;
Nanopartikel
;
Fullerene
;
Electronic books
;
Electronic books
