UID:
almahu_9948026427502882
Umfang:
1 online resource (597 p.)
ISBN:
1-281-76850-2
,
9786611768508
,
0-08-086005-2
Serie:
Methods of experimental physics ; v. 20
Inhalt:
Biophysics
Anmerkung:
Includes bibliographical references and indexes.
,
Front Cover; Biophysics; Copyright Page; Contents; Contributors; Publisher's Foreword; Foreword; Preface; List of Volumes in Treatise; Chapter 1. Nuclear Magnetic Resonance; 1.1. Introduction; 1.2. Phenomenon of Magnetic Resonance; 1.3. Spin Relaxation; 1.4. Experimental Methods; 1.5. Selected Studies on Biological Systems; Chapter 2. Nitroxide Spin Labels; 2.1. Introduction; 2.2. Spin-Lable Theory: A Descriptive Treatment; 2.3. Spin-Label Theory: A Mathematical Treatment; 2.4. Use of Spin Labels as Antigenic Determinants Capable of Reporting Their Physical State.
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Chapter 3. Raman Spectroscopy3.1. Introduction; 3.2. Origin of the Raman Spectrum; 3.3. Analysis of the Raman Spectrum; 3.4. Resonance Raman Scattering; 3.5. Instrumentation; 3.6. Strategy of Raman Spectroscopic Applications in Biology; 3.7. Conformational Studies; 3.8. Kinetic Studies; 3.9. Nonlinear Phenomena; 3.10. The Raman Microscope; 3.11. Conclusions and Prognostications; Chapter 4. Picosecond Laser Spectroscopy; 4.1. Introduction; 4.2. Nanosecond and Picosecond Spectroscopy; 4.3. Applications to Photosynthesis and Vision
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Appendix . Nonlinear Optical Phenomena, Optical Elements, and Detectors Related to Techniques of Picosecond SpectroscopyChapter 5. Fluorescence Methods for Studying Membrane Dynamics; 5.1. Introduction; 5.2. Molecular Rotation in Membranes; 5.3. Macroscopic Membrane Motions; 5.4. Applications; 5.5. Recent Developments; Chapter 6. Structure Determination of Biological Macromolecules Using X-Ray Diffraction Analysis; 6.1. Introduction; 6.2. Diffraction by a General Object; 6.3. Crystallography; 6.4. Protein Crystallography; 6.5. Fibers; Chapter 7. Laser Light Scattering; 7.1. Introduction
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7.2. Theory7.3. Instrumentation and General Techniques; 7.4. Diffusion Coefficients; 7.5. Large Particles; 7.6. Determination of Electrophoretic Mobilities; 7.7. Motility Measurements; 7.8. Applications in Cell Biology; 7.9. Blood Flow; 7.10. Gels and Solutions of Fibrillous Proteins; Chapter 8. Small-Angle Scattering Techniques for the Study of Biological Macromolecules and Macromolecular Aggregates; 8.1. Introduction; 8.2. The Experimental Problem; 8.3. Data Analysis; 8.4. Concluding Remarks; Chapter 9. Electron Microscopy; 9.1. Electron Microscopy as a Tool for Structure Determination
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9.2. Image Formation in the Electron Microscope9.3. Contrast Transfer Function Theory; 9.4. Three-Dimensional Reconstruction; 9.5. Radiation Damage; 9.6. Specimen Hydration within the Vacuum of the Instrument; 9.7. Recent Innovations in Experimental Methods; Chapter 10. Voltage Clamping of Excitable Membranes; 10.1. Introduction; 10.2. General Principles of Voltage Clamp; 10.3. Axial-Wire Voltage Clamp; 10.4. Voltage Clamp with Microelectrodes; 10.5. Voltage Clamp of an Isolated Patch Using External Pipettes; 10.6. Voltage Clamp with Gap Isolation Techniques; 10.7. Concluding Remarks
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10.A. Appendix
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English
Weitere Ausg.:
ISBN 0-12-475962-9
Sprache:
Englisch
