UID:
almafu_9959000033802883
Umfang:
1 online resource (382 p.)
Ausgabe:
1st ed. 1996.
ISBN:
1-280-83665-2
,
9786610836659
,
1-59259-544-8
Serie:
Methods in Molecular Biology, 57
Anmerkung:
Description based upon print version of record.
,
Site-Directed Mutagenesis Using Positive Antibiotic Selection -- In Vitro Site-Directed Mutagenesis Using the Unique Restriction Site Elimination (USE) Method -- Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates -- Site-Directed Mutagenesis Using a Uracil-Containing Phagemid Template -- Oligonucleotide-Directed Mutagenesis Using an Improved Phosphorothioate Approach -- Analysis of Point Mutations by Use of Amber Stop Codon Suppression -- A Simple Method for Site-Directed Mutagenesis with Double-Stranded Plasmid DNA -- Double-Stranded DNA Site-Directed Mutagenesis -- Solid-Phase In Vitro Mutagenesis Using a Plasmid DNA Template -- Targeted Mutagenesis Mediated by the Triple Helix Formation -- A Universal Nested Deletion Method Using an Arbitrary Primer and Elimination of a Unique Restriction Site -- Ordered Deletions Using Exonuclease III -- Ligase Chain Reaction for Site-Directed In Vitro Mutagenesis -- PCR-Based Site-Directed Mutagenesis -- In Vitro Recombination and Mutagenesis by Overlap Extension PCR -- Site-Directed Mutagenesis Using Overlap Extension PCR -- Modification of the Overlap Extension Method for Extensive Mutagenesis on the Same Template -- Site-Directed Mutagenesis In Vitro by Megaprimer PCR -- Using PCR for Rapid Site-Specific Mutagenesis in Large Plasmids -- PCR-Assisted Mutagenesis for Site-Directed Insertion/Deletion of Large DNA Segments -- Site-Directed Mutagenesis Using a Rapid PCR-Based Method -- A Simple Method to Introduce Internal Deletions or Mutations into Any Position of a Target DNA Sequence -- A Simple Method for Site-Specific Mutagenesis that Leaves the Rest of the Template Unaltered -- Multiple Site-Directed Mutagenesis -- Construction of Linker-Scanning Mutations by Oligonucleotide Ligation -- Construction of Linker-Scanning Mutations Using PCR -- Use of Codon Cassette Mutagenesis for Saturation Mutagenesis -- Saturation Mutagenesis by Mutagenic Oligonucleotide-Directed PCR Amplification (Mod-PCR) -- Random Mutagenesis of Short Target DNA Sequences via PCR with Degenerate Oligonucleotides -- Random Sequence Mutagenesis for the Generation of Active Enzymes -- Random Mutagenesis by Using Mixtures of dNTP and dITP in PCR -- PCR-Mediated Chemical Mutagenesis -- Oligonucleotide-Directed Random Mutagenesis Using the Phosphorothioate Method -- An Efficient Random Mutagenesis Technique Using an E. coli Mutator Strain.
,
English
Weitere Ausg.:
ISBN 0-89603-332-5
Sprache:
Englisch
