UID:
almahu_9949251338102882
Umfang:
1 online resource (X, 254 p. 9 illus.)
Ausgabe:
1st ed. 1995.
ISBN:
1-280-83648-2
,
9786610836482
,
1-59259-525-1
Serie:
Methods in Molecular Biology, 38
Inhalt:
The storage of biological material for regular or future use is a fundamental requirement in many biological and medical sciences. Cryopreservation and freeze-drying are the preferred techniques for achieving long-term storage, and have been applied to a diverse range of biological materials. Though the basis for many methodologies is common, laboratories frequently lack expertise with the correct storage procedures, so that many apply outdated or inappropriate protocols for storing their samples or cultures. Cryopreservation and Freeze-Drying Protocols is a compilation of the many and varied methodologies that have been developed in expert laboratories. The protocols are reproducible, robust, and in most instances have been transferred quite successfully to other laboratories. Our intended readers are those proposing to establish or improve biostorage systems in their own laboratories or units, whether concerned with culture collections, animal husbandry, aquaculture, or human fertilization programs. Because the emphasis of Cryopreservation and Freeze-Drying Protocols is on methodology, it is our intention to provide readers with the tools to make practical progress without reference to other sources. Each chapter deals with an organelle, cell, or tissue type: a short int- duction on the status of its biostorage development is followed by a detailed description of the materials required and a methodological p- tocol to be followed, with explanatory notes. This is very much a first edition; we hope and trust that future editions will contain cryopreservation and freeze-drying protocols for ceils, tissues, and organs that are at present still recalcitrant to succe- ful preservation.
Anmerkung:
Bibliographic Level Mode of Issuance: Monograph
,
Overview -- Virus Cryopreservation and Storage -- Freeze-Drying and Cryopreservation of Bacteria -- Freeze-Drying of Yeasts -- Cryopreservation of Yeast Cultures -- Cryopreservation and Freeze-Drying of Fungi -- Cryopreservation of Pathogenic and Nonpathogenic Free-Living Amoebae -- Long-Term Cryopreservation of Thylakoid Membranes -- Cryopreservation of Algae -- Cryopreservation of Plant Protoplasts -- A Two-Step or Equilibrium Freezing Procedure for the Cryopreservation of Plant Cell Suspensions -- Vitrification of Plant Cell Suspensions -- Cryopreservation of Shoot-Tips and Meristems -- Cryopreservation of Seeds -- Cryopreservation of the Sperm of the Pacific Oyster Crassostrea gigas -- Cryopreservation of Fish Spermatozoa -- Cryopreservation of Avian Spermatozoa -- Cryopreservation of Animal and Human Cell Lines -- Cryopreservation of Semen from Domestic Livestock -- Cryopreservation of Mammalian Embryos -- Cryopreservation of Mammalian Embryos -- Cryopreservation of Human Gametes -- Cryopreservation of Human Red Blood Cells.
,
English
Weitere Ausg.:
ISBN 1-4899-4089-8
Weitere Ausg.:
ISBN 0-89603-296-5
Sprache:
Englisch
