Umfang:
Online-Ressource (XI, 338 p. 55 illus., 29 illus. in color, digital)
ISBN:
9781461448150
Serie:
AAPS Advances in the Pharmaceutical Sciences Series 4
Inhalt:
A vast number of diagnostic and therapeutic decisions are based on measuring blood concentrations of molecules, yet most biochemical and pharmacological events actually take place in the tissues. Microdialysis is a key semi-invasive sampling technique to measure in vivo drug penetration to the target site in humans, the method being feasible in virtually every organ. Authored by international experts in this cutting edge field, this book will provide a comprehensive overview of microdialysis and its application for measuring drug distribution in drug development.
Inhalt:
A vast number of diagnostic and therapeutic decisions are based on measuring blood concentrations of molecules, yet most biochemical and pharmacological events actually take place in the tissues. Microdialysis is a key semi-invasive sampling technique to measure in vivo drug penetration to the target site in humans, the method being feasible in virtually every organ. Authored by international experts in this cutting edge field, this book will provide a comprehensive overview of microdialysis and its application for measuring drug distribution in drug development
Anmerkung:
Description based upon print version of record
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2.2.2 In Vitro RecoveryRecovery2.2.3 In Vivo RecoveryRecovery; 2.2.4 Mathematical Framework; 2.3…In Vivo Methods to Obtain Quantitative MicrodialysisMicrodialysis Data; 2.3.1 Zero and Ultraslow Flow Rate Method; 2.3.2 No-Net-Flux or Difference Method; 2.3.3 Extended or Dynamic No-Net-Flux Method; 2.3.4 Internal Standard Technique; 2.3.5 Retrodialysis or Reverse Dialysis of the Drug; 2.3.6 Combined Retrodialysis by Drug and Calibrator; 2.3.7 Endogenous Reference Technique; 2.3.8 Some Method Comparisons; 2.3.9 Modified Ultraslow MicrodialysisMicrodialysis
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2.4…Considerations in Experimental Conditions2.4.1 Static and Pulsatile Pressure; 2.4.2 Anesthesia; 2.4.3 Blood Flow; 2.4.4 Transporter Functionality and MetabolismMetabolism; 2.4.5 Composition and Temperature of the Perfusate; 2.4.6 Interactions of Probe Material with Drug or Tissue; 2.5…Considerations in Data Analysis; 2.6…Conclusions; References; 3 Analytical Considerations for Microdialysis MicrodialysisSampling; Abstract; 3.1…Analytical Challenges of MicrodialysisMicrodialysis Sampling; 3.2…Analytical Techniques for Off-Line Analysis; 3.2.1 Liquid Chromatography
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Abstract
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Microdialysis in Drug Development; Preface; Contents; Contributors; Part I General Aspects; 1 Introduction to the Microdialysis Technology; Abstract; 1.1…Access to the Tissue Compartment; 1.2…Development of microD; 1.3…microD Methodology; 1.4…Rationale for microD in Drug Research and Development; 1.5…Examples of microD Use in Drug Research and Development; 1.6…Challenges in microD; References; 2 RecoveryRecovery and CalibrationCalibration Techniques: Toward Quantitative MicrodialysisMicrodialysis; Abstract; 2.1…Introduction; 2.2…RecoveryRecovery; 2.2.1 The Concept of RecoveryRecovery
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3.2.2 Capillary Electrophoresis3.3…Detection Methods; 3.3.1 UV--VIS Absorbance; 3.3.2 Fluorescence Detection; 3.3.3 Chemiluminescence; 3.3.4 Electrochemical; 3.3.5 Mass Spectrometry; 3.3.6 Enzyme Assay; 3.3.7 Derivatization Methods; 3.3.7.1 Pre-Capillary/Column Derivatization; 3.3.7.2 Online Derivatization; 3.4…Online Versus Off-Line Analysis of MicrodialysisMicrodialysis Samples; 3.4.1 Online Coupling of MicrodialysisMicrodialysis to Liquid Chromatographic Analysis; 3.4.2 Online Coupling of Microdialysis to Capillary Electrophoresis; 3.4.3 Mass Spectrometry; 3.5…Microchip Electrophoresis
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3.6…SummaryReferences; 4 Regulatory Aspects of Microdialysis: A United States Food and Drug Administration Perspective; Abstract; 4.1…Background; 4.2…Role of Microdialysis in Assessing Bioavailability; 4.3…MicrodialysisMicrodialysis in Bioequivalence Assessment; 4.4…Experimental Factors Affecting the Outcome of Microdialysis; 4.4.1 Physicochemical Properties of the Analyte; 4.4.2 Factors Related to the Microdialysis Technique; 4.4.3 Physiological Factors; 4.5…Conclusions; References; Part II Specific Topics; 5 MicrodialysisMicrodialysis in CNS PKPD Research: Unraveling Unbound Concentrations
Weitere Ausg.:
ISBN 9781461448143
Weitere Ausg.:
Buchausg. u.d.T. ISBN 978-1-461-44814-3
Sprache:
Englisch
DOI:
10.1007/978-1-4614-4815-0
URL:
Volltext
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