Umfang:
1 Online-Ressource (246 Seiten, 12697 KB)
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Illustrationen, Diagramme
Inhalt:
Biomolecules such as proteins and lipids have vital roles in numerous cellular functions, including biomolecule transport, protein functions, cellular homeostasis and biomembrane integrity. Traditional biochemistry methods do not provide precise information about cellular biomolecule distribution and behavior under native environmental conditions since they are not transferable to live cell samples. Consequently, this can lead to inaccuracies in quantifying biomolecule interactions due to potential complexities arising from the heterogeneity of native biomembranes. To overcome these limitations, minimal invasive microscopic techniques, such as fluorescence fluctuation spectroscopy (FFS) in combination with fluorescence proteins (FPs) and fluorescence lipid analogs, have been developed. FFS techniques and membrane property sensors enable the quantification of various parameters, including concentration, dynamics, oligomerization, and interaction of biomolecules in live cell samples. In this work, several FFS approaches and membrane ...
Anmerkung:
kumulative Dissertation
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Dissertation Universität Potsdam 2023
Weitere Ausg.:
Erscheint auch als Druck-Ausgabe Petrich, Annett Quantitative fluorescence microscopy methods to investigate molecular interactions and dynamics in living cells Potsdam, 2023
Sprache:
Englisch
Schlagwort(e):
Hochschulschrift
DOI:
10.25932/publishup-61180
URN:
urn:nbn:de:kobv:517-opus4-611800
