UID:
almahu_9947367401502882
Format:
1 online resource (507 p.)
Edition:
First edition.
ISBN:
0-12-801341-9
,
0-12-801218-8
Series Statement:
Methods in Enzymology ; Volume 551
Content:
Two new volumes of Methods in Enzymology continue the legacy of this premier serial with quality chapters authored by leaders in the field. Circadian Rhythms and Biological Clocks Part A and Part B is an exceptional resource for anybody interested in the general area of circadian rhythms. As key elements of timekeeping are conserved in organisms across the phylogenetic tree, and our understanding of circadian biology has benefited tremendously from work done in many species, the volume provides a wide range of assays for different biological systems. Protocols are provided to assess clock fun
Note:
Description based upon print version of record.
,
Front Cover; Circadian Rhythms and Biological Clocks, A; Copyright; Contents; Contributors; Preface; Part I: Organismal Rhythms as Read-Outs of Clock Function; Chapter 1: Studying Circadian Rhythm and Sleep Using Genetic Screens in Drosophila; 1. Introduction: Studying Circadian Behavior in the Fruit Fly, Drosophila melanogaster; 2. Screening for Circadian Rhythm and Sleep Mutants; 2.1. History of circadian rhythm screens; 2.2. History of sleep screens; 3. Screening Techniques; 3.1. EMS mutagenesis; 3.1.1. X-linked EMS screen for sleep mutants; 3.2. Transposon mutagenesis
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3.3. Tools for conditional transgene expression3.4. Drosophila RNAi libraries and screens; 3.4.1. RNAi screen for suppressors and enhancers of shaggy; 3.4.2. Neuronal RNAi screen for sleep mutants; 3.5. Advantages and drawbacks of screening with RNAi in comparison to chemical and transposon mutagenesis; Acknowledgments; References; Chapter 2: Dissecting the Mechanisms of the Clock in Neurospora; 1. Introduction; 1.1. Methods of analysis of circadian rhythms in Neurospora crassa; 1.2. Circadian rhythms in other fungi; 2. Molecular Mechanism of the Neurospora Circadian Oscillator
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3. Core Clock Components3.1. The FRQ/FRH complex; 3.2. The White Collar Complex; 3.3. The input and output of the clock; 4. Conclusion; References; Chapter 3: High-Throughput and Quantitative Approaches for Measuring Circadian Rhythms in Cyanobacteria Using Bioluminescence; 1. Theory; 2. Build a Computer-Controlled Turntable; 2.1. Materials; 2.2. Programs; 2.3. Protocol; 3. Use a Computer-Controlled Turntable; 3.1. Programs; 3.2. Protocol; 4. Analyzing Data from Turntable; 4.1. Programs; 4.2. Protocol; 5. Steps to Extract Reliable Quantitative Information from Bioluminescence Levels
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5.1. Equipment5.2. Programs; 5.3. Protocol; Acknowledgments; References; Chapter 4: Using Circadian Entrainment to Find Cryptic Clocks; 1. Introduction; 1.1. Entrainment protocols; 1.1.1. Photoperiod-Longer or shorter days, shorter or longer nights; 1.1.2. Zeitgeber strength; 1.1.3. Dawn and dusk transitions; 1.1.4. T cycles and phase angles; 2. Methods; 2.1. Saccharomyces cerevisiae; 2.1.1. Identification of the optimal dilution rate; 2.1.2. T cycles; 2.1.3. Zeitgeber strength and entrainment of yeast; 2.1.4. Constant conditions: Free-running rhythm?; 2.2. Caenorhabditis elegans
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3. DiscussionAcknowledgments; References; Chapter 5: Wavelet-Based Analysis of Circadian Behavioral Rhythms; 1. Introduction; 2. Fourier and Wavelet Methods for Time Series Analysis; 2.1. Discrete Fourier transform; 2.1.1. Background and theory; 2.1.2. Applications to chronobiology; 2.2. Short-time Fourier transform; 2.2.1. Background and theory; 2.2.2. Applications to chronobiology; 2.3. Analytic wavelet transform; 2.3.1. Background and theory; 2.3.2. Applications to chronobiology; 2.4. Discrete wavelet transform; 2.4.1. Background and theory; 2.4.2. Applications to chronobiology
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2.5. Example with wavelet analysis of a behavioral record
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English
Language:
English
