UID:
almahu_9949697923302882
Format:
1 online resource (601 p.)
ISBN:
1-281-01136-3
,
9786611011369
,
0-08-049707-1
Series Statement:
Methods in enzymology, v. 364
Content:
Nuclear Receptors are inducible transcription factors that mediate complex effects on development, differentiation and homeostasis. They regulate the transcription of their target genes through binding to DNA sequences.*Analysis of Nuclear Receptor Ligands*Structure/Function Analysis of Nuclear Receptors*Analysis of Nuclear Receptor Co-Factors and Chromatin Remodeling
Note:
Description based upon print version of record.
,
Front Cover; Nuclear Receptors; Copyright Page; Table of Contents; Contributor to Volume 364; Preface; Volumes in Series; Section I: Analysis of Nuclear Receptor Ligands; Chapter 1. Identification of Novel Nuclear Hormone Receptor Ligands by Activity-Guided Purification; Chapter 2. Quantitation of Receptor Ligands by Mass Spectrometry; Chapter 3. Site-Specific Fluorescent Labeling of Estrogen Receptors and Structure-Activity Relationships of Ligands in Terms of Receptor Dimer Stability; Chapter 4. Cell-Free Ligand Binding Assays for Nuclear Receptors
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Chapter 5. Design and Synthesis of Receptor LigandsSection II: Structure/Function Analysis of Nuclear Receptors; Chapter 6. Bioinformatics of Nuclear Receptors; Chapter 7. Application of Random Peptide Phage Display to the Study of Nuclear Hormone Receptors; Chapter 8. Methods for Detecting Domain Interactions in Nuclear Receptors; Chapter 9. The Orphan Receptor SHP and the Three-Hybrid Interference Assay; Chapter 10. Regulation of Glucocorticoid Receptor Ligand- Binding Activity by the hsp90/hsp70-based Chaperone Machinery; Chapter 11. Analysis of Receptor Phosphorylation
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Section III: Analysis of Nuclear Receptor Cofactors and Chromatin RemodelingChapter 12. Acetylation and Methylation in Nuclear Receptor Gene Activation; Chapter 13. Steroid Receptor Coactivator Peptidomimetics; Chapter 14. Biochemical Isolation and Analysis of a Nuclear Receptor Corepressor Complex; Chapter 15. Isolation and Functional Characterization of the TRAP/Mediator Complex; Chapter 16. Study of Nuclear Receptor-Induced Transcription Complex Assembly and Histone Modification by Chromatin Immunoprecipitation Assays
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Section IV: Identification of Nuclear Receptor Target Genes and EffectorsChapter 17. Serial Analysis of Gene Expression and Gene Trapping to Identify Nuclear Receptor Target Genes; Chapter 18. Expression Cloning of Receptor Ligand Transporters; Chapter 19. Nuclear Receptor Target Gene Discovery Using High-Throughput Chromatin Immunoprecipitation; Chapter 20. RNA Gel Shift Assays for Analysis of Hormone Control of mRNA Stability; Chapter 21. Expression Cloning of Ligand Biosynthetic Enzymes; Section V: Use of Animal Models to Study Nuclear Receptor Function
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Chapter 22. Targeted Conditional Somatic Mutagenesis in the Mouse: Temporally-Controlled Knock Out of Retinoid Receptors in Epidermal KeratinocytesChapter 23. Analysis of Small Molecule Metabolism in Zebrafish; Chapter 24. Analysis of Nuclear Receptor Function in the Mouse Auditory System; Chapter 25. Analysis of Estrogen Receptor Expression in Tissues; Chapter 26. In Vivo and In Vitro Reporter Systems for Studying Nuclear Receptor and Ligand Activities; Chapter 27. Methods to Characterize Drosophila Nuclear Receptor Activation and Function In Vivo; Author Index; Subject Index
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Color Plates Section
,
English
Additional Edition:
ISBN 0-12-182267-2
Language:
English
