ISSN:
1873-4766
Content:
The transfer of substances via the blood-placenta barrier is tightly regulated and critical for the fetus and the expecting mother. In case of necessary medical interventions during pregnancy a comprehensive knowledge about the interaction of the drugs with this barrier is indispensable. Therefore well-engineered test systems are needed and valuable transport systems are helpful. We developed an in vitro microfluidic blood-placenta barrier system consisting of the human trophoblast cell line BeWo and human primary placental pericytes. The integrity and stability of the model was verified by a permeability assay and immunocytochemistry. As potential drug carriers magnetic nanoparticles with various coatings were applied and their ability to pass the barrier was quantified by magnetic particle spectroscopy. We could demonstrate that up to 4% of negatively charged nanoparticles pass the barrier in a time-dependent manner.
In:
Journal of magnetism and magnetic materials, Amsterdam : North-Holland Publ. Co., 1975, Volume 521 (2021), part 2, 167535, 1873-4766
In:
volume:521
In:
year:2021
In:
number:2
In:
day:1
In:
month:03
In:
elocationid:167535
Language:
English
DOI:
10.1016/j.jmmm.2020.167535
URL:
Volltext
(lizenzpflichtig)
Author information:
Hochhaus, Andreas 1959-
Author information:
Mosig, Alexander S.
Author information:
Friedrich, Ralf P. 1966-
Author information:
Zahn, Diana
Author information:
Dutz, Silvio 1972-
Author information:
Raasch, Martin 1987-