UID:
almafu_9959328578802883
Format:
1 online resource (259 pages)
ISBN:
0470849681
,
9780470849682
,
0470011122
,
9780470011126
,
0470011114
,
9780470011119
Uniform Title:
Biocalorimetry.
Note:
Revised edition of: Biocalorimetry.
,
Cover Contents Preface List of Contributors Part I General Introduction 1 Applications of Biocalorimetry: Binding, Stability and Enzyme Kinetics 1.1 Introduction 1.2 Principles of isothermal titration calorimetry (ITC) 1.3 Applications of ITC in the life sciences 1.4 Thermodynamic signatures of non-covalent interactions 1.5 Thermodynamic discrimination (TD) 1.6 ITC as a tool for studying drug DNA interactions 1.7 ITC as a tool for studying protein DNA interactions 1.8 The application of calorimetry for examining hydration effcts 1.9 The use of ITC for studying the kinetics and thermodynamics of enzyme catalysis 1.10 Principles of differential scanning calorimetry (DSC) 1.11 Applications of DSC in the life sciences 1.12 Thermodynamic stability 1.13 Shelf life versus thermodynamic stability 1.14 Specific and non-specific binding 1.15 Intrinsic and extrinsic macromolecular stability 1.16 Oligomerization 1.17 The use of DSC for examining nucleic acid helix coil transitions 1.18 Summary Acknowledgements References Part II Isothermal Titration Calorimetry 2 Isothermal Titration Calorimetry: A Tutorial 2.1 Introduction 2.2 Thermodynamic characterization 2.3 Instrumentation 2.4 Raw data 2.5 Basic considerations for experimental set-up 2.6 Data analysis 2.7 Summary Application notes Acknowledgement References 3 The Application of Isothermal Titration Calorimetry to Drug Discovery 3.1 Introduction 3.2 Overview of the drug discovery process 3.3 Experimental measurement of thermodynamic binding parameters 3.4 ITC in drug discovery 3.5 Summary References 4 Dissecting the Thermodynamics of DNA Protein Interactions 4.1 Introduction 4.2 Model systems 4.3 Comparison with the hydrophobic effect 4.4 Protonation and charged charged hydrogen bonds 4.5 Dissection of the binding entropy 4.6 Entropy contributions to the Sso7d DNA interaction 4.7 Entropy contributions to the GCN4 DNA interaction 4.8 Discussion Acknowledgements References 5 Salt Effects in Ribonuclease Ligand Interactions: Screening or Competitive Binding? 5.1 Introduction 5.2 Anion binding to a protein protein complex 5.3 Charge charge interactions in ribonuclease binding 5.4 Conclusions Acknowledgement References 6 Thermodynamics Structure Correlations of Sulfonamide Inhibitor Binding to Carbonic Anhydrase 6.1 Introduction 6.2 Identification of protonation reactions occurring upon binding 6.3 Observed thermodynamics of inhibitor binding to CA 6.4 Energetics of inhibitor protonation 6.5 Sulfonamide anion binding thermodynamics 6.6 Correlations between structures and the thermodynamics of sulfonamide binding to CA 6.7 Conclusions References 7 Energetics of the Interaction of Human Acidic Fibroblast Growth Factor with Heparin and the Functional Analogue Myo-Inositol Hexasulfate 7.1 Introduction 7.2 Thermodynamic parameter derived from ITC experiments 7.3 Discussion Acknowledgement References 8 Thermodynamics of SH2 Domain Binding 8.1 Introduction 8.2 SH2 domains and human pathologies 8.3 Structure and ligand binding 8.4 SH2-domain-binding inhibitors 8.5 Conclusions References 9 Titration Calorimetry as a Tool to Determine Thermodynamic and Kinetic Parameters of Enzymes 9.1 Introduction 9.2 ICT of enzyme catalysed reactions 9.3 Summary Acknowledgements References Part III Differential Scanning Calorimetry 10 Energetics of Site-Specific DNA Recognition by Integrase Tn916 10.1 Introduction 10.2 Conformational stability of INT-DBD and the target DNA duplex 10.3 Thermodynamics of complex formation measured by titration calorimetry 10.4 Thermal dissociation and unfolding of the protein DNA complex 10.5 The heat capacity change of protein DNA association 10.6 Discussion References 11 Linkage Between Temperature and Chemical Denaturant Effects on Protein Stability: the Interpretation of Calorimetrically-Determined m Va.
Additional Edition:
Print version: Biocalorimetry II. Chichester ; Hoboken, NJ : John Wiley & Sons, ©2004 ISBN 0470011114
Language:
English
Subjects:
Chemistry/Pharmacy
Keywords:
Electronic books.
;
Electronic books.
;
Konferenzschrift
;
Electronic books.
URL:
https://onlinelibrary.wiley.com/doi/book/10.1002/0470011122
URL:
https://onlinelibrary.wiley.com/doi/book/10.1002/0470011122
URL:
Volltext
(URL des Erstveröffentlichers)
URL:
https://onlinelibrary.wiley.com/doi/book/10.1002/0470011122
