UID:
almahu_9948025863902882
Format:
1 online resource (119 p.)
ISBN:
0-12-407860-5
Content:
Approaches to Research on the Systematics of Fish-Borne Trematodes is a concise guide for systematic studies of the prevalence of fish-borne trematodes both in the endemic areas and experimental laboratories. It includes methods to identify species of fish-borne trematodes to enhance the precision of research studies based on the metacercarial stage. Misidentification of trematode species is a common occurrence when researchers are new to the field and have no guidance. Consequentially, sometimes publications report inaccurate prevalence rates of these parasites. This compact
Note:
Description based upon print version of record.
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Front Cover; Approaches to Research on the Systematics of Fish-Borne Trematodes; Copyright Page; Contents; Preface; 1 Medically Important Fish-Borne Zoonotic Trematodes; 1.1 Major fish-borne Trematodes; 1.1.1 Opisthorchiidae; 1.1.2 Treatment of Liver Flukes; 1.2 Other fish-borne Trematodes; 1.2.1 Clinostomatidae; 1.2.2 Echinostomatidae; 1.2.3 Heterophyidae; 1.2.4 Troglotrematidae; References; 2 Collection of Fish-Borne Trematodes from the Final Host; 2.1 Diagnostic Methods; 2.1.1 Stool Egg Examination; 2.1.2 Copro-DNA Detection; 2.1.3 Antibody Detection; 2.1.4 Worm Finding
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2.2 Treatment and Purgation2.3 Collection of Adult FBT from Animals; 2.4 Worm Preparation for Morphological Study; 2.5 Diagnostic Characteristics of FBT; 2.5.1 Family Clinostomatidae Luhe, 1901; 2.5.2 Family Echinostomatidae Looss, 1902; 2.5.3 Family Heterophyidae Leiper, 1909; 2.5.4 Family Opisthorchiidae Braun, 1901; 2.5.5 Family Troglotrematidae Odhner, 1914; References; 3 Collection of Fish-Borne Trematode Cercaria; 3.1 Snail Host of Fish-Borne Trematodes; 3.1.1 Family Bithyniidae; 3.1.2 Family Planorbidae; 3.1.3 Family Thiaridae; 3.1.4 Family Semisulcospiridae
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3.2 Cercaria Morphological Study3.3 Morphology of Fresh Cercaria; 3.4 Vital Stain; 3.5 Chaetotaxy; 3.6 Polymerase Chain Reaction; References; 4 Collection of Fish-Borne Trematodes in Infective Stage from the Fish: The Second Intermediate Host; 4.1 Development of Metacercariae; 4.2 Methods of Fish Examination for Metacercariae; 4.2.1 Compression Method; 4.2.2 Digestion Method; 4.3 Mobile Laboratory for Fish Examination; 4.4 Method of Excysting Metacercariae; 4.5 Morphology of Metacercariae; 4.5.1 Clinostomatidae; 4.5.2 Echinostomatidae; 4.5.3 Heterophyidae; 4.5.4 Opisthorchiidae
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4.5.4.1 Life Span of Metacercariae4.5.4.2 Infectivity of Metacercariae; 4.5.5 Troglotrematidae; References; 5 Molecular Systematics of Fish-Borne Trematodes; 5.1 What is Molecular Systematics?; 5.2 Species Identification: Morphological and Molecular Approaches; 5.3 Advantages and Disadvantages of using DNA Sequences for Systematics; 5.4 Molecular Markers; 5.5 The Regions of DNA used for Molecular Systematics; 5.5.1 Nuclear Coding Region; 5.5.2 Ribosomal DNA; 5.5.3 Mitochondrial DNA; 5.6 Molecular Systematics of fish-borne Trematodes
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5.6.1 Opisthorchiidae Looss 1899 and Heterophyidae Leiper 19095.6.2 Clinostomidae Lühe, 1901; 5.7 Summary and Future Direction; References; 6 Methods of Molecular Study: DNA Sequence and Phylogenetic Analyses; 6.1 Criterion for Selecting Appropriate Genetic Markers; 6.2 Phylogenetic Analysis; 6.3 How to Construct Phylogenetic Trees; 6.3.1 Assembling a Dataset; 6.3.2 Multiple Sequence Alignment; 6.3.3 Determination of Substitution Model; 6.3.4 Tree Building; 6.3.5 Tree Evaluation; 6.4 Further Perspectives; References; Appendix A; References; Appendix B; 1 Genomic DNA Preparation
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1.1 Preservation of Tissue Sample in Ethanol
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English
Additional Edition:
ISBN 0-12-407720-X
Language:
English
