UID:
almafu_9958129965702883
Format:
1 online resource (683 p.)
Edition:
3rd ed., Part A.
ISBN:
1-281-02106-7
,
9786611021061
,
0-08-052252-1
Series Statement:
Methods in cell biology, v. 63
Content:
Each chapter presents a detailed background of the described method, its theoretical foundations, and its applicability to different biomedical material. Updated chapters describe either the most popular methods or those processes that have evolved the most since the past edition. Additionally, a large portion of the volume is devoted to clinical cytometry. Particular attention is paid to applications of cytometry in oncology, the most rapidly growing area.Key Features * Contains 56 extensive chapters authored by world authorities on cytometry* Covers a wide range of topics, in
Note:
Previous ed.: 1994.
,
Front Cover; Methods in Cell Biology; CONTENTS; Contents of Volume 64; Contributors; Preface to the Third Edition; Preface to the Second Edition; Preface to the First Edition; Part I: Principles of Cytometry and General Methods; Chapter 1. A Brief History of Flow Cytometry and Sorting; I. Introduction; II. Instrumentation; III. Applications; References; Chapter 2. Principles of Flow Cytometry: An Overview; I. Introduction; II. The Illumination of a Particle; III. Fluidics: Centering Particles in the Illuminating Beam; IV. Collection of Light Signals from Particles
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V. From Light Signals to a Data FileVI. From Data to Information; VII. Sorting; VIII. Conclusions; References; Chapter 3. Laser Scanning Cytometry; I. Introduction; II. Background; III. Description of the Instrument; IV. The Utility and Operational Characteristics of Some Laser Scanning Cytometry List Mode Features; V. Utility of Solid Phase Cytometry for Cell Preparation; VI. Future Directions; References; Chapter 4. Principles of Confocal Microscopy; I. Brief History of Microscope Development; II. Development of Confocal Microscopy; III. Image Formation in Confocal Microscopy
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IV. Useful Fluorescent Probes for Confocal MicroscopyV. Applications of Confocal Microscopy; VI. Conclusions; References; Chapter 5. Optical Measurements in Cytometry: Light Scattering, Extinction, Absorption, and Fluorescence; I. Introduction; II. Signal Processing Tasks in Flow Cytometry: An Overview; III. The Optical Signal: Interaction of Light with Cells; IV. Detection: Converting Optical Signals to Current; V. Electronics: Converting Current to Voltage; VI. Fluorescence Compensation and Logarithmic Amplification; VII. Peak Detection, Integration, and Pulse Width Measurement. Triggering
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VIII. Measurement Sensitivity: Changing Concepts and the Bottom LineReferences; Chapter 6. Flow Cytometric Fluorescence Lifetime Measurements; I. Introduction; II. Applications of the Technology; III. Cell Preparation and Staining; IV. Fluorescence Lifetime Flow Cytometry Instrumentation; V. Results; VI. Critical Aspects of the Technology; VII. Future Directions; References; Chapter 7. Principles of Data Acquisition and Display; I. Introduction; II. Pulse Characterization Using Analog and Hybrid Circuits; III. Analog Data Display; IV. Analog-to-Digital Conversion
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V. Pulse Characterization by Digital Signal ProcessingVI. Data Storage and Display with Digital Computers; References; Chapter 8. Time as a Flow Cytometric Parameter; I. Introduction; II. Historical Overview; III. Sample Mixing and Delivery; IV. Data Analysis; V. Applications; VI. Conclusions; References; Chapter 9. Protein Labeling with Fluorescent Probes; I. Introduction; II. Labeling of Proteins with Organic Fluorescent Dyes; III. Labeling of Proteins with Phycobiliproteins; IV. Conclusion; References; Part II: Cell Preparation; Chapter 10. Preparation of Cells from Blood; I. Introduction
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II. Collection, Transport, and Storage of Blood
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English
Additional Edition:
ISBN 0-12-544166-5
Language:
English
