UID:
edoccha_9960073096002883
Format:
1 online resource (xi, 176 pages) :
,
illustrations (some color).
ISBN:
1-299-39845-6
,
1-4377-3474-X
Series Statement:
Micro & nano technologies series
Content:
Engineered Nanopores for Bioanalytical Applications is the first book to focus primarily on practical analytical applications of nanopore development. These nanoscale analytical techniques have exciting potential because they can be used in applications such as DNA sequencing, DNA fragment sizing, DNA/protein binding, and protein/protein binding. This book provides a solid professional reference on nanopores for readers in academia, industry and engineering and biomedical fields. In addition, the book describes the instrumentation, fabrication, and experimental methods necess
Note:
Description based upon print version of record.
,
Front Cover; Engineered Nanopores for Bioanalytical Applications; Copyright Page; List of Contributors; Contents; Introduction; Engineered nanopores for bioanalytical applications; 1 Ion Transport in Nanopores; 1.1 Introduction; 1.2 Brownian motion; 1.3 Net transport of ions: the Nernst-Planck equation and its derivation; 1.4 The conductance of a pore with uncharged walls; 1.4.1 Cylindrical pores; 1.4.2 Pores with noncylindrical geometries; 1.4.3 Access resistance; 1.5 The effect of surface charge; 1.5.1 Charged surfaces in solution; 1.5.2 The conductance of nanopores with charged inner walls
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1.5.3 The ζ-potential of colloids and charged particles1.5.4 Electroosmosis-fluid motion close to a charged wall in response to an external electric field; 1.6 Particle translocation through nanopores-the model of deBlois and Bean; 1.6.1 Small spheres solution; 1.6.2 "Broad range" solution; References; 2 DNA Translocation; 2.1 Introduction; 2.2 Physics of a polyelectrolyte inside a nanopore; 2.2.1 Electrostatic potential around a charged surface; 2.2.1.1 Nanopore; 2.2.1.2 Polyelectrolyte; 2.3 Electroosmotic flow inside a cylindrically nanopore; 2.4 DNA inside a nanopore
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2.4.1 Free translocation2.5 Capture rate and probability of successful translocation; 2.5.1 Dominating effects; 2.5.1.1 Diffusion-limited regime; 2.5.1.2 Drift regime; 2.5.1.3 Free energy barrier; 2.5.2 Discussion of successful translocation; 2.6 Stalling DNA in a nanopore; 2.6.1 Silicon nitride nanopore with optical tweezers; 2.7 Stalling DNA in nanocapillaries; 2.7.1 Electrostatic characterization; 2.7.2 Force measurements inside glass capillaries; References; 3 Instrumentation for Low-Noise High-Bandwidth Nanopore Recording; 3.1 Introduction
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3.2 Components of a nanopore setup and their integration3.2.1 Nanopore support structure; 3.2.2 Fluidic cell; 3.2.3 Ag/AgCl electrodes; 3.2.4 Noise pickup; 3.3 Low-current measurement techniques; 3.3.1 Shunt resistor; 3.3.2 Resistive feedback; 3.3.3 Capacitive feedback; 3.4 Bandwidth and background noise; 3.4.1 Low-frequency spectrum; 3.4.1.1 Thermal noise; 3.4.1.2 Shot noise; 3.4.1.3 Flicker noise; 3.4.1.4 Protonation noise; 3.4.2 High-frequency spectrum; 3.4.2.1 Dielectric noise; 3.4.2.2 Input capacitance noise; 3.5 Noise filtering, sampling, and resolution
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3.6 Outlook: pushing the detection limit3.6.1.1 Solid-state nanopore devices with reduced capacitance; 3.6.1.2 Integrated nanopores; Ackowledgements; References; 4 Biological Pores on Lipid Bilayers; 4.1 Introduction; 4.2 Formation: overview and experimental protocols; 4.3 Pore characterization: overview and experimental protocols; 4.3.1 Electrophysiological approaches; 4.3.1.1 Critical dimensions; 4.3.1.2 Selectivity; 4.3.2 High-resolution structures; 4.4 Bacterial pore-forming toxins; 4.4.1 α-Hemolysin; 4.4.2 Anthrax protective antigen; 4.5 Bacterial porins; 4.5.1 Outer membrane porins
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4.5.2 Mycobacterium smegmatis: MspA
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English
Additional Edition:
ISBN 1-4377-3473-1
Language:
English
