Kooperativer Bibliotheksverbund

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Format: 1 online resource (351 p.)

ISBN: 0-12-810317-5 , 0-12-800465-7

Content: Laboratory Techniques in Rabies Diagnosis, Research and Prevention provides a basic understanding of the current trends in rabies. It establishes a new facility for rabies surveillance, vaccine and antibody manufacturing. It offers clarity about the choice of laboratory methods for diagnosis and virus typing, of systems for producing monoclonal and polyclonal antibodies and of methods for testing potency of vaccines and antibodies. The book covers advancements in the classical methods described as well as recent methods and approaches pertaining to rabies diagnosis and research. Supplies techniques pertaining to rabies diagnosis and research Provides an update on the conventional and modern vaccines for rabies prevention Offers updates on the full length antibodies and antibody fragments for post exposure prophylaxis of rabies Presents technique descriptions that can be used to be compared to industry protocols to identify and establish potential new techniques.

Note: Bibliographic Level Mode of Issuance: Monograph , Front Cover -- Current Laboratory Techniques in Rabies Diagnosis, Research and Prevention -- Copyright Page -- Contents -- Foreword -- List of Contributors -- ONE: INTRODUCTION -- 1. Basic Facts about Lyssaviruses -- 1.1 Introduction -- 1.2 Virion and Genome Organization -- 1.3 Phylogeny and Serologic Cross-Reactivity of Lyssaviruses -- 1.4 Host Range -- 1.5 Pathobiology -- References -- TWO: RABIES DIAGNOSIS -- A: Demonstration of Viral Subunits and Antigens -- 2. Demonstration of Lyssavirus Antigens by a Direct Rapid Immunohistochemical Test -- 2.1 Introduction -- 2.2 Materials -- 2.2.1 Reagents -- 2.2.2 Equipment -- 2.2.3 Biological Material: Deterioration or Decomposition -- 2.3 Methods -- 2.3.1 Performing Diagnostic Assay -- 2.3.2 Reading and Recording of Results -- 2.3.3 Test Results are Reported if the Following Observations are Made -- 2.4 Discussion -- References -- 3. Demonstration of Rabies Virus Antigens by a Latex Agglutination Test -- 3.1 Introduction -- 3.2 Materials -- 3.2.1 Reagents -- 3.2.2 Biological Materials -- 3.2.3 Equipment -- 3.3 Methods -- 3.3.1 Production of Anti-Rabies Serum -- 3.3.2 Preparation of Anti-Rabies IgG -- 3.3.3 Latex Coating -- 3.3.4 Test Saliva -- 3.3.5 Slide Agglutination -- 3.4 Discussion -- 3.4.1 Experimental Tips -- 3.4.2 Technical Notes -- 3.4.3 Critical Parameters -- References -- 4. Rabies Diagnosis: Demonstration of Viral Antigens by Flow Cytometry -- 4.1 Introduction -- 4.2 Materials -- 4.2.1 Reagents -- 4.2.2 Equipment -- 4.2.3 Biological Materials -- 4.3 Methods -- 4.3.1 Infection and Incubation -- 4.3.2 Cell Staining -- 4.3.3 Flow Cytometric Analysis of Infection -- 4.3.4 Interpretation of Results -- 4.4 Discussion -- 4.4.1 Experimental Tips -- 4.4.2 Critical Parameters and Troubleshooting -- 4.4.3 Precautions -- 4.4.4 Alternative Materials and Methods -- 4.4.5 Time Considerations. , 4.4.6 Limitations -- 4.4.7 Future Considerations -- Acknowledgments -- References -- 5. Demonstration of Rabies Virus Antigens by an Immunochromatographic Strip Test -- 5.1 Introduction -- 5.2 Materials -- 5.2.1 Reagents -- 5.2.2 Biological Materials -- 5.2.3 Materials and Equipment -- 5.3 Methods -- 5.3.1 Colloidal Gold Conjugation -- 5.3.2 Test Strip Preparation -- 5.3.3 Application of the Test Strip -- 5.4 Discussion -- 5.4.1 Experimental Tips -- 5.4.2 Technical Notes -- 5.4.3 Future Considerations -- References -- B: Demonstration of Viral Nucleic Acids -- 6. Demonstration of African Lyssavirus RNA with Real-Time Polymerase Chain Reaction -- 6.1 Introduction -- 6.2 Materials -- 6.2.1 Reagents -- 6.2.2 Equipment -- 6.2.3 Biological Material -- 6.3 Methods -- 6.3.1 Preparation of Reagent Master Mix -- 6.3.2 Cycling Protocol -- 6.3.2.1 Analysis of Results -- 6.4 Discussion -- References -- 7. Real-Time Quantitative Polymerase Chain Reaction for the Demonstration of Lyssavirus Nucleic Acid -- 7.1 Real-Time Quantitative Polymerase Chain Reaction for the Demonstration of Lyssavirus Nucleic Acid -- 7.2 Methodology -- 7.2.1 Viral RNA Extraction -- 7.2.2 Generic Pan Lyssavirus Real-Time Polymerase Chain Reactions -- 7.2.3 Real-Time Polymerase Chain Reaction for Lyssavirus Species using Specific Taqman Probes -- 7.3 Discussion -- Acknowledgments -- References -- 8. Reverse Transcription-Loop-Mediated Isothermal Amplification System for the Detection of Rabies Virus -- 8.1 Introduction -- 8.2 Materials -- 8.2.1 Reagents -- 8.2.2 Primer Set for Reverse Transcription-Loop-Mediated Isothermal Amplification -- 8.2.3 Equipment -- 8.2.4 Biological Materials and Sample Preparation -- 8.3 Methods -- 8.3.1 Preparation of Mastermix -- 8.3.2 Mixing of Mastermix and Sample Solution -- 8.3.3 Amplification Reaction -- 8.3.4 Detection and Interpretation of Results. , 8.4 Discussion -- Acknowledgments -- References -- 9. Detection of Viral Nucleic Acids by In Situ Hybridization -- 9.1 Introduction -- 9.2 Materials -- 9.2.1 Reagents -- 9.2.2 Equipment -- 9.2.3 Biological Materials -- 9.2.4 Laboratory Animals -- 9.3 Methods -- 9.3.1 Generation of Control Tissues -- 9.3.2 Section Preparation -- 9.3.3 Production and Evaluation of Probe -- 9.3.4 In Situ Hybridization -- 9.3.5 Interpretation of Results -- 9.4 Discussion -- 9.4.1 Experimental Tips -- 9.4.2 Critical Parameters, Troubleshooting, and Precautions -- 9.4.3 Alternative Materials and/or Methods -- 9.4.4 Time Considerations -- 9.4.5 Limitations -- 9.4.6 Future Considerations -- Acknowledgments -- References -- 10. Genetic Characterization via Pyrosequencing -- 10.1 Introduction -- 10.2 Materials -- 10.2.1 Reagents and Equipment -- 10.2.2 Diagnostic Samples -- 10.3 Methods -- 10.3.1 Preliminary Phase - Population of the Local Database (IdentiFire Library) -- 10.3.2 Phase 1 - RNA Extraction, One-Step RT-PCR, Gel Electrophoresis Analysis -- 10.3.3 Phase 2 - Pyrosequencing Reaction and Result Analysis -- 10.4 Discussion -- 10.4.1 Experimental Tips -- 10.4.2 Critical Parameters and Troubleshooting -- 10.4.3 Precautions -- 10.4.4 Alternative Methods -- 10.4.5 Time Consideration -- 10.4.6 Limitations -- 10.4.7 Future Considerations -- Acknowledgments -- References -- C: Demonstration of Viral Antibodies and Immune Complexes -- 11. Demonstration of Immune Complexes by Capture Enzyme-Linked Immunosorbent Assay -- 11.1 Introduction -- 11.2 Materials -- 11.2.1 Reagents -- 11.2.2 Equipment and Other Consumables -- 11.2.3 Biological Materials -- 11.3 Methods -- 11.4 Discussion -- 11.4.1 Experimental Tips -- 11.4.2 Precautions -- 11.4.3 Alternative Materials and/or Methods -- 11.4.4 Limitations and Future Considerations -- Acknowledgments -- References. , 12. Demonstration of Viral Antibodies by an Immunochromatographic Strip Test -- 12.1 Introduction -- 12.2 Materials -- 12.2.1 Reagents -- 12.2.2 Equipment -- 12.3 Methods -- 12.4 Discussion -- Acknowledgments -- References -- 13. Rabies Diagnosis: Demonstration of Viral Antibodies by Flow Cytometry -- 13.1 Introduction -- 13.2 Materials -- 13.2.1 Reagents -- 13.2.2 Equipment -- 13.2.3 Biological Materials -- 13.3 Methods -- 13.3.1 Major Step 1 -- 13.3.2 Major Step 2 -- 13.3.3 Interpretation of Results and Determination of Rabies Virus-Neutralizing Antibodies Levels -- 13.4 Discussion -- 13.4.1 Experimental Tips -- 13.4.2 Critical Parameters and Troubleshooting -- 13.4.3 Precautions -- 13.4.4 Alternative Materials and/or Methods -- 13.4.5 Time Considerations -- 13.4.6 Limitations -- 13.4.7 Future Considerations -- Acknowledgments -- References -- 14. Demonstration of Rabies Antibody by a Latex Agglutination Test -- 14.1 Introduction -- 14.2 Materials -- 14.2.1 Reagents -- 14.2.2 Biological Materials -- 14.2.3 Equipment -- 14.3 Methods -- 14.3.1 Latex Coating -- 14.3.2 Slide Agglutination -- 14.4 Discussion -- 14.4.1 Experimental Tips -- 14.4.2 Technical Notes -- References -- 15. Demonstration of Viral Antibodies by Pseudotype Virus Neutralization -- 15.1 Introduction -- 15.2 Materials -- 15.2.1 Reagents -- 15.2.2 Equipment -- 15.2.3 Biological Materials -- 15.3 Methods -- 15.3.1 Generation of Pseudotyped Virus Stock -- 15.3.2 Titration of Pseudotyped Virus Stock -- 15.3.3 Setting Up a PVNA -- 15.3.4 Reporter Gene Assay -- 15.3.5 Interpretation of Results -- 15.4 Discussion -- 15.4.1 Experimental Tips and Critical Parameters -- 15.4.2 Limitations -- 15.4.3 Future Considerations -- Acknowledgments -- References -- D: Typing/Differentiation of Lyssaviruses -- 16. Sanger Sequencing of Lyssaviruses -- 16.1 Background -- 16.2 Methodology. , 16.2.1 Preparing Polymerase Chain Reaction Amplicons for the ABI Genetic Analyzers -- 16.2.2 Purifying the Polymerase Chain Reaction Product -- 16.2.3 Big Dye Terminator Cycle Sequencing Reactions -- 16.2.4 Ethanol Precipitation of Sequencing Reactions -- 16.2.5 Preparing 96-Well Sample Plate(s) for the Genetic Analyzers -- 16.2.6 Loading 96-Well Sample Plate(s) -- 16.2.7 Checking Run Reagents -- 16.2.8 Starting the Run on the 3130xl -- 16.2.9 Starting the Run on the 3730 -- 16.2.10 Analyzing Output Data -- 16.3 Discussion -- Acknowledgments -- References -- 17. Next Generation Sequencing of Lyssaviruses -- 17.1 Background -- 17.2 Methodology -- 17.2.1 Viral RNA Extraction -- 17.2.2 Preparation of cDNA for Sequencing on the Roche 454 FLX(+) Titanium -- 17.2.3 Analyzing and Annotating 454 Genome Sequences -- 17.2.4 Depth of Coverage Analysis -- 17.3 Discussion -- Acknowledgments -- References -- 18. Genetic Characterization of Rabies Viruses by In Situ Hybridization -- 18.1 Introduction -- 18.2 Materials -- 18.3 Methods -- 18.3.1 Generation of Control Tissues -- 18.3.2 Section Preparation -- 18.3.3 Production and Evaluation of Probes -- 18.3.4 In Situ Hybridization -- 18.3.5 Interpretation of Results -- 18.4 Discussion -- 18.4.1 Experimental Tips -- 18.4.2 Alternative Materials and Methods -- 18.4.3 Future Considerations -- Acknowledgments -- References -- 19. Oligonucleotide Microarray: Applications for Lyssavirus Speciation -- 19.1 Introduction -- 19.2 Methodology -- 19.2.1 Oligonucleotide Design -- 19.2.2 Microarray Fabrication: In-House Printed Microarray -- 19.2.3 Microarray Fabrication: In Situ Synthesis (Inkjet Printing) Technology -- 19.2.4 Nucleic Acid Extraction and DNase Digestion -- 19.2.5 Reverse Transcription-Polymerase Chain Reaction -- 19.2.6 Labeling Amplified Target -- 19.2.7 Microarray Hybridization. , 19.2.8 Post Hybridization Washes and Image Capture. , English

Additional Edition: ISBN 0-12-800014-7

Additional Edition: ISBN 1-322-01792-1

Language: English