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Format: 1 online resource (166 pages)

ISBN: 0-12-821570-4

Content: Stem cell therapy is a fast-growing field of medicine with remarkable prospects in a broad spectrum of diseases. Stem Cell Therapy: Practical Considerations addresses the biological properties of stem cells, mechanisms of action; as well as actual therapeutic decisions such as cell type, source, dose, manipulation, and route of injection.

Note: Front Cover -- Stem Cell Therapy -- Copyright Page -- Dedication -- Contents -- I. Stem cells: basic biology -- 1 Introduction -- 1.1 Establishing the concept of stem cells -- Further reading -- 2 Anatomy and Histology -- 2.1 Blood supply -- 2.2 Niche -- 2.3 Hemopoietic stem cell niches -- 2.4 Differences between osteoblastic and vascular niches -- 2.5 Components of the hemopoietic stem cell niche -- 2.6 Mesenchymal stem cell niche -- 2.6.1 Bone marrow mesenchymal stem cell niche -- 2.7 Mesenchymal stem niche in adipose tissue -- 2.7.1 Epidermal stem cell niche -- 2.7.2 Intestinal epithelial niche -- 2.8 Niche functions -- 2.9 Regulation of niche function -- Further reading -- 3 Stem cells: definition, biological types, classifications, and properties -- 3.1 Definition -- 3.2 Classification -- 3.3 Properties -- 3.3.1 Potency -- 3.3.1.1 Totipotent stem cells -- 3.3.1.2 Pluripotent stem cells -- 3.3.1.3 Molecular determinants of pluripotency -- 3.3.1.4 Cell cycle profile of pluripotency -- 3.3.1.5 Multipotent stem cells -- 3.3.1.6 Oligopotent stem cells -- 3.3.1.7 Bipotent stem cells -- 3.3.1.8 Unipotent stem cells -- 3.3.2 Self-renewal -- 3.3.2.1 Regulation of self-renewal -- 3.3.3 Stem cell plasticity -- 3.3.4 Evidence of stem cell transdifferentiation -- 3.3.5 Theories of transdifferentiation -- 3.3.5.1 Requirements for proof of transdifferentiation -- 3.3.5.2 Factors affecting frequency of transdifferentiation -- 3.3.5.3 Induction of transdifferentiation -- 3.3.5.4 Evaluation of transdifferentiation -- 3.4 Differentiation -- 3.5 Homing and engraftment -- 3.5.1 Definition -- 3.5.2 Mechanism of homing -- 3.5.3 Enhanced and directed homing -- 3.5.4 Stem cell trafficking -- 3.5.5 Mechanism of stem cell trafficking -- 3.5.6 Role of stem cell trafficking in regeneration -- Further reading. , II. Stem cell induced regeneration: principles and applications -- 4 Mechanisms of stem cell-related regeneration -- 4.1 Regulation of tissue regeneration -- 4.2 Overview of the mechanism of regeneration -- 4.2.1 Transdifferentiation -- 4.2.2 Contribution to tissue regeneration -- 4.2.3 Variation in transdifferentiation potential -- 4.2.4 Molecular mechanisms of transdifferentiation -- 4.3 Signaling pathways involved in stem cell transdifferentiation -- Mogrify algorithm -- 4.4 Activation of endogenous repair system -- 4.5 Induction of angiogenesis -- 4.5.1 Variability in angiogenic potential of mesenchymal stem cells -- 4.6 Secretory function -- 4.6.1 Microvesicles and exosomes -- 4.6.2 Paracrine action -- 4.7 Mitochondrial transfer -- 4.7.1 Inducers of transfer -- 4.7.2 Mechanisms -- 4.7.3 Effects -- 4.7.4 Variability in mitochondrial transfer according to mesenchymal stem cell source -- 4.8 Microenvironment modulation -- 4.9 Conclusion -- Further reading -- 5 Sources of stem cells for regenerative purposes -- 5.1 Characteristics of the ideal source of stem cells -- 5.2 Sources of mesenchymal stem cells -- 5.3 Skin mesenchymal stem cells -- 5.4 Periodontal ligament stem cells -- 5.4.1 Sources of hemopoietic stem cells -- 5.5 Cord blood -- 5.5.1 Sources of endothelial cells -- 5.6 Tissue-specific stem cells -- Further reading -- 6 Basic concepts in stem cell therapy: stem cell therapy strategy development -- 6.1 Introduction -- 6.2 What is the basic defect? -- 6.2.1 Cellular defects -- 6.2.2 Structural defects -- 6.3 Which cell lineage is the best for repair? Choosing optimum cell lineage -- 6.4 What is the best source for a cell type? Choosing the optimum cell source -- 6.4.1 Hemopoietic stem cells -- 6.4.2 Mesenchymal stem cells -- 6.5 What is the optimum laboratory protocol? -- 6.6 Choosing the optimum route of injection. , 6.7 Factors affecting homing of stem cells -- 6.7.1 Methods to improve stem cell homing -- 6.7.2 Mesenchymal stem cell homing -- 6.8 Optimal timing for cell therapy -- 6.9 Optimum number of cells for injection -- 6.10 Choosing the method of evaluation -- Further reading -- III. Guidelines for stem cell therapy -- 7 Stem Cell Therapy: Laboratory guidelines -- 7.1 Introduction -- 7.1.1 Regulatory bodies -- 7.1.1.1 Stem cell therapy types in regard to manipulation -- 7.2 Laboratory guidelines -- 7.2.1 Structural facilities -- 7.2.1.1 Good tissue practice -- 7.2.1.1.1 Good tissue practice environmental control -- 7.2.1.2 Good manufacturing practice -- 7.2.1.2.1 Facilities -- 7.2.1.2.2 Equipment -- 7.2.1.2.3 Basic equipment for stem cell laboratory are -- 7.2.1.2.4 Supplies and reagents -- 7.2.1.2.5 Processing and process control -- 7.2.2 Personnel qualifications and job description -- 7.2.2.1 Administrative team -- 7.2.2.2 Ethics team -- 7.2.2.3 Clinical evaluation team -- 7.2.2.4 Stem cell collection team -- 7.2.2.5 Stem cell handling team (laboratory) -- 7.2.2.6 Clinical followup team -- 7.3 Quality control team -- Further reading -- 8 Protocol guidelines for clinical decisions in stem cell therapy -- 8.1 Introduction -- 8.1.1 Timing of intervention -- 8.1.2 Stem cell injection -- 8.1.2.1 Route of injection -- 8.1.3 Frequency of injection -- 8.1.3.1 Methods of evaluation -- 8.1.3.1.1 Patient -- 8.1.3.1.2 Cells -- Reference -- Further reading -- 9 Protocol guidelines: laboratory decisions in stem cell therapy -- 9.1 Introduction -- 9.2 Choosing the cell purification level -- 9.3 Choice of purification strategy -- 9.4 Choice of stem cell differentiation status -- 9.5 Choice of cell dose -- 9.6 Choice of tracking method -- 9.6.1 Methods of in vivo cell tracking -- 9.6.1.1 Experimental -- 9.6.1.2 Methods of in vivo tracking in humans. , 10 Phases of translational stem cell research -- 10.1 Introduction -- 10.2 Laboratory experiments -- 10.3 Preclinical, experimental -- 10.3.1 Value of animal experiments -- 10.3.1.1 Animal models -- 10.3.1.2 Species selection -- 10.3.1.3 Strain selection -- 10.3.1.4 Methods of inducing animal models -- 10.3.2 Small versus large animals -- 10.3.3 Challenges in animal experiments -- 10.3.4 Ethical considerations -- 10.4 Clinical trials -- Further reading -- 11 Ethical guidelines of stem cell therapy -- 11.1 Introduction -- 11.1.1 Ethical guidelines for the use of embryonic cells -- 11.1.2 Ethical concerns -- 11.1.3 Ethical guidelines for preclinical studies -- 11.1.4 Principles of ethical guidelines of clinical translation -- Further reading -- 12 Stem cell therapy: practical examples -- 12.1 Neuroregeneration -- 12.1.1 In vitro experiments -- 12.2 Experimental animal study -- 12.2.1 Induction of animal model -- 12.2.2 Stem cell therapy -- 12.2.3 Followup -- 12.3 Results -- 12.3.1 Clinical translational study -- 12.4 Results of clinical translational study -- 12.5 Replacement of defective protein -- 12.5.1 Epidermolysis bullosa -- 12.6 Results -- 12.7 Multifactorial defects -- 12.7.1 Results -- 12.7.1.1 In vitro experiments -- 12.7.1.2 Clinical results -- 12.8 Conclusion -- Further reading -- Appendix A -- A.1 Standard operating procedures for stem cell therapy procedures -- A.1.1 Collection -- A.1.1.1 Collection of peripheral blood hemopoietic stem cells -- A.1.1.2 Collection of bone marrow hemopoietic stem cells -- A.1.1.3 Cord blood stem cell collection -- A.1.1.4 Collection of stem cells from adipose tissue -- A.1.2 Isolation -- A.1.2.1 Mononuclear cell separation by density gradient centrifugation -- A.1.2.2 Mesenchymal Stem Cell Isolation from Wharton Jelly -- A.1.3 Mesenchymal stem cell culture -- A.1.3.1 Identification of mesenchymal stem cells. , A.1.4 Differentiation -- A.1.4.1 Plating cells for differentiation -- A.1.4.2 Evaluation of differentiation -- A.1.5 Coating of flasks -- A.1.6 Labeling -- Index -- Back Cover.

Additional Edition: Print version: Gabr, Hala M. Stem Cell Therapy San Diego : Elsevier Science & Technology,c2022 ISBN 9780128215692

Language: English

Keywords: Llibres electrònics