Cancer Letters, 2007, Vol.250(1), pp.107-116
The efficacy of Onconase on the growth of a panel of chemosensitive and chemoresistant neuroblastoma cell lines was investigated. Onconase decreased cell viability of chemosensitive (IMR-32, UKF-NB-3) and chemoresistant neuroblastoma cell lines characterised by high expression of P-glycoprotein (P-gp) (UKF-NB-3 DOX ) or by high P-gp expression in combination with mutated p53 (UKF-NB-3 VCR , Be(2)-C), in a similar manner. Moreover, Onconase caused cell cycle block in G1 phase and induced caspase-independent cell death. Transmission electron microscope investigations suggested that Onconase-induced autophagy contributes to Onconase-induced cell death. Antitumour activity of Onconase against naïve and drug-resistant neuroblastoma xenografts was confirmed in animals.
Onconase ; Neuroblastoma ; Multi-Drug-Resistance ; P-Glycoprotein ; P53 ; Medicine
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