Format:
1 Online-Ressource (146 Seiten, 12329 KB)
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Illustrationen, Diagramme
Content:
With Saccharomyces cerevisiae being a commonly used host organism for synthetic biology and biotechnology approaches, the work presented here aims at the development of novel tools to improve and facilitate pathway engineering and heterologous protein production in yeast. Initially, the multi-part assembly strategy AssemblX was established, which allows the fast, user-friendly and highly efficient construction of up to 25 units, e.g. genes, into a single DNA construct. To speed up complex assembly projects, starting from sub-gene fragments and resulting in mini-chromosome sized constructs, AssemblX follows a level-based approach: Level 0 stands for the assembly of genes from multiple sub-gene fragments; Level 1 for the combination of up to five Level 0 units into one Level 1 module; Level 2 for linkages of up to five Level 1 modules into one Level 2 module. This way, all Level 0 and subsequently all Level 1 assemblies can be carried out simultaneously. Individually planned, overlap-based Level 0 assemblies enable scar-free and ...
Note:
Dissertation (kumulativ) Universität Potsdam 2017
Additional Edition:
Erscheint auch als Druck-Ausgabe Hochrein, Lena Development of a new DNA-assembly method and its application for the establishment of a red light-sensing regulation system Potsdam, 2017
Language:
English
Keywords:
Hochschulschrift
URN:
urn:nbn:de:kobv:517-opus4-404441
URL:
https://nbn-resolving.org/urn:nbn:de:kobv:517-opus4-404441
URL:
https://d-nb.info/121840311X/34
Author information:
Müller-Röber, Bernd 1964-
