Format:
Online-Ressource
,
v.: digital
Edition:
Online-Ausg. Springer eBook Collection. Biomedical and Life Sciences Electronic reproduction; Available via World Wide Web
ISBN:
9783642046087
,
1282830988
,
9781282830981
,
9783642046094
Content:
This concise yet comprehensive guide to the methods and protocols of immunohistochemistry covers established techniques and current developments in the field such as the use of epitope tags, multiple immunolabeling and diagnostic immunohistochemistry.
Content:
While many excellent texts and monographs exist which cover various aspects of immunohistochemistry, the lack of a concise comprehensive guide to using the field's methods was a major motivation for the authors to write this book.The authors carefully selected the methods and easy-to-adopt protocols discussed. In addition to estabished techniques, special attention was paid to current developments in immunohistochemistry such as antigen retrieval, signal amplification, the use of epitope tags, multiple immunolabeling, and diagnostic immunohistochemistry. All procedures examined have been tested by the authors, many are routinely used in daily practice in their institute. Each chapter starts with a short introduction to the respective method's underlying principles before presenting step-by-step protocols and instructions, including precise recipes for all materials needed. Thus, the book is suitable for a broad audience, ranging from students and technical assistants to experienced researchers.
Note:
Includes bibliographical references and index
,
Chapter 1: Antibodies for Immunohistochemistry; Chapter 2: Antibody Labeling and the Choice of the Label; Chapter 3: Probes Processing in Immunohistochemistry; Chapter 4: Working with Antibodies; 5: Background Staining, Autofluorescence and Blocking Steps; Chapter 6: Immunostaining Enhancement; Chapter 7: Multiple Multicolor Immunoenzyme Staining; Chapter 8: Multiple Immunofluorescence Staining; Chapter 9: Antigen Detection on Tissues Using Primary Antibody Raised in the Same Species; Chapter 10: Probes for Staining Specific Cellular Organelles
,
Chapter 11: The Use of Epitope Tags in HistochemistryChapter 12: Immunohistochemistry at the Ultrastructural Level; Chapter 13: Diagnostic Immunohistochemistry; Chapter 14: A Picture Is Worth a Thousand Words; Buckwala_Index_o.pdf
,
Electronic reproduction; Available via World Wide Web
Additional Edition:
ISBN 9783642046094
Additional Edition:
ISBN 9783642046087
Additional Edition:
ISBN 1282830945
Additional Edition:
Buchausg. u.d.T. Buchwalow, Igor B. Immunohistochemistry Heidelberg : Springer, 2010 ISBN 9783642046087
Language:
English
Subjects:
Biology
Keywords:
Immuncytochemie
;
Immuncytochemie
DOI:
10.1007/978-3-642-04609-4
URL:
Volltext
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