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  • 1
    In: Nature Reviews Microbiology, 2012, Vol.10(9), p.618
    Description: A comprehensive understanding of host-pathogen interactions requires a knowledge of the associated gene expression changes in both the pathogen and the host. Traditional, probe-dependent approaches using microarrays or reverse transcription PCR typically require the pathogen and host cells to be physically separated before gene expression analysis. However, the development of the probe-independent RNA sequencing (RNA-seq) approach has begun to revolutionize transcriptomics. Here, we assess the feasibility of taking transcriptomics one step further by performing 'dual RNA-seq', in which gene expression changes in both the pathogen and the host are analysed simultaneously.
    Keywords: Host-Pathogen Interactions ; Gene Expression Profiling -- Methods ; Sequence Analysis, RNA -- Methods;
    ISSN: 1740-1526
    E-ISSN: 17401534
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