Format:
Online-Ressource
ISSN:
1423-0100
Content:
Introduction: The rapid emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants and their potential to endangering the global health has increased the demand for a fast-tracking method in comparison to the next-generation sequencing (NGS) as a gold standard assay, particularly in developing countries. This study was designed to evaluate the performance of a commercial multiplex real-time PCR technique (GA SARS-CoV-2 OneStep RT-PCR Kit, Iran) for identification of SARS-CoV-2 variants of concern (VOCs) compared to the Oxford Nanopore NGS assay. Methods: A total of 238 SARS-CoV-2-positive respiratory samples from different waves of COVID-19 in Iran were randomly selected in this study. To determine the SARS-CoV-2 VOC, the samples were analyzed via the commercial triple target assay, GA SARS-CoV-2 OneStep RT-PCR Kit, and NGS as well. Results: The results revealed good concordance between GA SARS-CoV-2 OneStep RT-PCR Kit and NGS for identification of SARS-CoV-2 VOCs. GA SARS-CoV-2 OneStep RT-PCR Kit identified Wuhan, Alpha, and Delta variants with 100% relative sensitivity and specificity. Regarding Omicron subvariants of BA.1, BA.2, and BA.4/5, the relative sensitivity of 100%, 100%, and 81.5% and the relative specificity of 95.3%, 93.5%, and 100% were observed. Conclusion: Overall, GA SARS-CoV-2 OneStep RT-PCR Kit can be used as a rapid and cost-effective alternative to NGS for identification of SARS-CoV-2 VOCs.
In:
volume:66
In:
number:1
In:
year:2023
In:
pages:136-141
In:
extent:6
In:
Intervirology, Basel : Karger, 1973-, 66, Heft 1 (2023), 136-141 (gesamt 6), 1423-0100
Language:
English
URN:
urn:nbn:de:101:1-2023122800253329000322
URL:
https://doi.org/10.1159/000534067
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023122800253329000322
URL:
https://d-nb.info/131475517X/34
URL:
https://karger.com/int/article-pdf/66/1/136/4083555/000534067.pdf
URL:
https://karger.com/doi/10.1159/000534067
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