Format:
Online-Ressource
ISSN:
1460-2075
Content:
Salmonella enterica requires a type III secretion system, designated Spi/Ssa, to survive and proliferate within macrophages. The Spi/Ssa system is encoded within the SPI‐2 pathogenicity island and appears to function intracellularly. Here, we establish that the SPI‐2‐encoded SpiC protein is exported by the Spi/Ssa type III secretion system into the host cell cytosol where it interferes with intracellular trafficking. In J774 macrophages, wild‐type Salmonella inhibited fusion of Salmonella‐containing phagosomes with lysosomes and endosomes, and interfered with trafficking of vesicles devoid of the microorganism. These inhibitory activities required living Salmonella and a functional spiC gene. Purified SpiC protein inhibited endosome–endosome fusion in vitro. A Sindbis virus expressing the SpiC protein interfered with normal trafficking of the transferrin receptor in vivo. A spiC mutant was attenuated for virulence, suggesting that the ability to interfere with intracellular trafficking is essential for Salmonella pathogenesis.
In:
volume:18
In:
number:14
In:
year:1999
In:
pages:3924-3933
In:
extent:10
In:
European Molecular Biology Organization, The EMBO journal, Heidelberg : EMBO Press, 1982-, 18, Heft 14 (1999), 3924-3933 (gesamt 10), 1460-2075
Language:
English
DOI:
10.1093/emboj/18.14.3924
URN:
urn:nbn:de:101:1-2023120605024751841170
URL:
https://doi.org/10.1093/emboj/18.14.3924
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023120605024751841170
URL:
https://d-nb.info/1312277955/34
URL:
https://doi.org/10.1093/emboj/18.14.3924
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