Format:
1 Online-Ressource (115 Seiten, 4614 KB)
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Content:
Scope: Several studies show that excessive lipid intake can cause hepatic steatosis. To investigate lipotoxicity on cellular level, palmitate (PA) is often used to highly increase lipid droplets (LDs). One way to remove LDs is autophagy, while it is controversially discussed if autophagy is also affected by PA. It is aimed to investigate whether PA-induced LD accumulation can impair autophagy and punicalagin, a natural autophagy inducer from pomegranate, can improve it. Methods and results: To verify the role of autophagy in LD degradation, HepG2 cells are treated with PA and analyzed for LD and perilipin 2 content in presence of autophagy inducer Torin 1 and inhibitor 3-Methyladenine. PA alone seems to initially induce autophagy-related proteins but impairs autophagic-flux in a time-dependent manner, considering 6 and 24 h PA. To examine whether punicalagin can prevent autophagy impairment, cells are cotreated for 24 h with PA and punicalagin. Results show that punicalagin preserves expression of autophagy-related proteins and ...
Note:
Dissertation Universität Potsdam 2022
Additional Edition:
Erscheint auch als Druck-Ausgabe Korovila, Ioanna Role of proteolytic systems in lipotoxicity - induced hepatocellular damage Potsdam, 2021
Language:
English
Keywords:
Hochschulschrift
DOI:
10.25932/publishup-55238
URN:
urn:nbn:de:kobv:517-opus4-552385
URL:
https://doi.org/10.25932/publishup-55238
URL:
https://nbn-resolving.org/urn:nbn:de:kobv:517-opus4-552385
URL:
https://d-nb.info/1263644422/34
Author information:
Schwerdtle, Tanja 1975-
Author information:
Grune, Tilman 1962-
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