Format:
xxi, 116 Seiten
,
Illustrationen, Diagramme
Content:
The mitochondrial chaperone complex HSP60/HSP10 facilitates mitochondrial protein homeostasis by folding more than 300 mitochondrial matrix proteins. It has been shown previously that HSP60 is downregulated in brains of type 2 diabetic (T2D) mice and patients, causing mitochondrial dysfunction and insulin resistance. As HSP60 is also decreased in peripheral tissues in T2D animals, this thesis investigated the effect of overall reduced HSP60 in the development of obesity and associated co-morbidities. To this end, both female and male C57Bl/6N control (i.e. without further alterations in their genome, Ctrl) and heterozygous whole-body Hsp60 knock-out (Hsp60+/-) mice, which exhibit a 50 % reduction of HSP60 in all tissues, were fed a normal chow diet (NCD) or a highfat diet (HFD, 60 % calories from fat) for 16 weeks and were subjected to extensive metabolic phenotyping including indirect calorimetry, NMR spectroscopy, insulin, glucose and pyruvate tolerance tests, vena cava insulin injections, as well as histological and molecular ...
Note:
Dissertation Universität Potsdam 2021
Additional Edition:
Erscheint auch als Online-Ausgabe Hauffe, Robert Investigating metabolic consequences of an HSP60 reduction during diet-induced obesity Potsdam, 2021
Language:
English
Keywords:
Hochschulschrift
Author information:
Schürmann, Annette 1960-
Author information:
Kleinridders, André 1978-
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