Format:
Online-Ressource (PDF-Datei: 95 S., 4284 KB)
,
Ill., graph. Darst.
Content:
Bauchspeicheldrüsenkrebs, Zelladhäsion, p120ctn
Content:
The aim of this study was to evaluate different expressions of p120ctn exon A and exon B in the example of patients with ductal pancreatic cancer. The differentiation of the isoforms was performed using antibodies against p120ctn exon A and exon B. Also specially manufactured p120ctn GFP fusion proteins showed the sensitivity and specificity of the antibodies in immunofluorescence and Western blot. Evaluation of the expression assigned two groups, one with dominant p120ctn exon A and one with dominant p120ctn exon B expression. In comparing the survival times are significantly worse found in the dominance of p120ctn exon A. When viewed from transfected cells in scratch assay Patu 8889S showed an increased migration from exon A p120ctn-transfected cells. Similarly, the proliferation is increased by the example of transfected HEK293 cells with p120ctn exon A. This is connected with redistribution of p120ctn in the cytosol and the nucleus especially at exon A p120ctn dominance. This leads on to the lower membrane binding impeding the formation of clusters of cadherins, the limited transport of E-cadherin and an increased degradation of E-cadherin. Instead, find p120ctn increased in the cytosol with modulation of the cytoskeleton. This leads to the increased migration. In the nucleus, it is the binding of the transcription factor Kaiso at p120ctn in the regulation of the Wnt signaling pathway. This is essential for embryonic development and active also in many malignant tumors ...
Note:
Greifswald, Univ., Diss., 2012
Additional Edition:
Erscheint auch als Druck-Ausgabe Budde, Christoph Untersuchung der unterschiedlichen Expression von Exon A und Exon B Isoformen des p120 Catenin bei Patienten mit duktalem Pankreaskarzinom 2011
Language:
German
Subjects:
Medicine
Keywords:
Bauchspeicheldrüsenkrebs
;
Zelladhäsion
;
Hochschulschrift
URN:
urn:nbn:de:gbv:9-001207-8
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