Format:
Online-Ressource
ISSN:
1522-2683
Content:
Abstract: A major limitation in the applicability of automated DNA sequencing instruments has been the difficulty in using user‐defined oligonucleotide primers which allow sequencing reactions to start at any specific point in a region of interest. Recently, new chemistries have become available for fluorescent labeling which will begin to facilitate the use of any oligonucleotide primer with automated DNA sequencers. In this report, we describe several methods for automated primerdirected DNA sequencing, and compare and discuss the relative merits and limitations of these methods.
In:
volume:13
In:
number:1
In:
year:2005
In:
pages:552-559
In:
extent:8
In:
Electrophoresis, Weinheim : Wiley-Blackwell, 1980-, 13, Heft 1 (2005), 552-559 (gesamt 8), 1522-2683
Language:
English
DOI:
10.1002/elps.11501301113
URN:
urn:nbn:de:101:1-2024011905210726310762
URL:
https://doi.org/10.1002/elps.11501301113
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2024011905210726310762
URL:
https://d-nb.info/1316445887/34
URL:
https://doi.org/10.1002/elps.11501301113
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