Format:
8
ISSN:
1742-2051
Content:
Determining interacting cellular partners of drugs by chemical proteomic techniques is complex and tedious. Most approaches rely on activity-based probe profiling and compound-centric chemical proteomics. The anti-malarial artemisinin also exerts profound anti-cancer activity, but the mechanisms of action are incompletely understood. In the present investigation, we present a novel approach to identify artemisinin-interacting target proteins. Our approach overcomes usual problems in traditional fishing procedures, because the drug was attached to a surface without further chemical modification. The proteins identified effect among others, cell cycle arrest, apoptosis, inhibition of angiogenesis, disruption of cell migration, and modulation of nuclear receptor responsiveness. Furthermore, a bioinformatic approach confirmed experimentally identified proteins and suggested a large number of other interacting proteins. Theoretically predicted interaction partners may serve as a starting point to complete the whole set of proteins binding artemisinin.
Note:
Gesehen am 25.06.2019
In:
Molecular BioSystems, Cambridge : Royal Society of Chemistry, 2005, 8(2012), 4, Seite 1311-1318, 1742-2051
In:
volume:8
In:
year:2012
In:
number:4
In:
pages:1311-1318
In:
extent:8
Language:
English
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