Format:
1 Online-Ressource (117 p)
ISBN:
9781493965403
Content:
Dedication -- Preface -- Contents -- Contributors -- Part 1: Rebranding Classical Protein Chemistry: Proteomics of the Past -- Chapter 1: Introduction: HIV-1 Proteomics, Why Should One Care? -- References -- Chapter 2: HIV-1 Biology at the Protein Level -- The Benefits of HIV-1 Protein Study -- Evolution of Retroviral Biochemistry -- HIV-1 Virus Genome and Its Proteins: Basics -- Viral Proteins in HIV-1 Virions -- HIV-1 Assembly: Why Gag Polyproteins? -- The HIV-1 Proteins -- Gag (http://www.uniprot.org/uniprot/P12493) -- MA, Matrix, p17MA -- CA, Capsid, or p24CA
Content:
SP1, Spacer Protein 1, p2Gag -- Nucleocapsid, NC, p7NC -- p6Gag -- GagPol (http://www.uniprot.org/uniprot/P12497) -- p6Pol, Preprotease, Transframe Protein -- PR, Protease -- RT, Reverse Transcriptase -- IN, Integrase -- Env (http://www.uniprot.org/uniprot/P03377) -- Accessory Proteins -- Vpr (http://www.uniprot.org/uniprot/P12520) -- Nef (http://www.uniprot.org/uniprot/P03406) -- Regulatory Proteins -- Incorporation of Cellular Proteins into HIV-1: Bystanders, Partners, Captives, and Assassins -- The Purity Problem -- HIV-1 Proteome Summary -- References
Content:
Chapter 3: 25 Years of HIV-1 Biochemistry -- Aspects of HIV-1 Particle Biochemistry -- HIV-1 Proteomics Before There Was Proteomics -- Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis -- Immunoblotting/Western Blotting -- Reversed-Phase High-Pressure Chromatography -- Automated N-Terminal (Edman Degradation) Protein Sequencing -- Amino Acid Analysis -- References -- Part 2: Modern HIV-1 Proteomics -- Chapter 4: Proteomic Studies of HIV-1 -- Introduction -- Proteomic Studies of HIV-1 -- Proteomics: "A Three-Legged Stool" -- Sample Preparation -- Protein and Peptide Quantitation
Content:
Sample Cleanup: Beware of Traditional Detergents -- Mass Spectrometry: The Basics -- Vacuum System and Source -- The Mass Analyzer -- The Detector -- Tandem Mass Spectrometry -- Mass Spectrometry in the Context of HIV-1 Proteomics -- Chromatography Considerations -- Mass Spectrometry Acquisition: Qualitative Versus Quantitative Methods -- Data-Dependent Analysis -- Label-Free Quantitative Approaches: Spectral Counting and Data-Independent Analysis -- Labeling Approaches -- Isobaric Tagging -- Stably Incorporated Labeled Amino Acids -- Informatics
Content:
Targeted HIV-1 Proteomics and the Path to Clinical Applications -- Selective Reaction Monitoring -- Quick Start Guide for SRM -- Thinking Back to Our Experiments and Motivations -- Alternative Approaches -- Affinity Pulldown Approaches -- Antigen Presentation -- Protein Arrays -- Conclusions -- References -- Chapter 5: HIV-1 Sequencing -- Introduction -- Reasons Why Understanding HIV/SIV Variation Is Important from a Biological and Proteomics Perspective -- The Evolution of HIV/SIV Sequencing Technologies -- HIV/SIV Deep Sequencing Methodologies -- Pre-experimental Questions
Content:
Sequencing Methodology
Note:
Description based upon print version of record
Additional Edition:
9781493965427
Additional Edition:
9781493965403
Additional Edition:
Print version Graham, David R HIV-1 Proteomics : From Discovery to Clinical Application New York, NY : Springer New York,c2016 9781493965403
Language:
English
Keywords:
Electronic books
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