UID:
kobvindex_GFZ20220428165152
Format:
XVIII, 165 Seiten
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Illustrationen, Diagramme
Note:
Kumulative Dissertation
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Dissertation, Universität Potsdam, 2021
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Table of Contents
Acknowledgements
Abstract
Zusammenfassung
List of figure
List of tables
List of abbreviation
Chapter 1
1. Introduction
1.1 Research background
1.1.1 Response of mountain plant diversity to climate change
1.1.2 Response of Arctic vegetation composition and diversity to climate change
1.1.3 Understanding the critical mechanisms of community assembly are essential for sustaining ecosystem services
1.1.4 Pollen analysis as a traditional tool for representing palaeovegetation
1.1.5. Sedimentary ancient DNA (sedaDNA) is a useful tool for Quaternary ecology tracking
1.2 Study area
1.3 Aims and objectives
1.4 Structure of the thesis
1.4.1 Overview of the chapter
1.4.2 Author's contributions
1.4.3 Methods
Chapter 2
2 Manuscript 1: Sedimentary ancient DNA reveals warming-induced alpine habitat loss threat to Tibetan Plateau plant diversity
2.1 Abstract
2.2 Introduction
2.3 Results and discussion
2.4 Methods
2.5 Acknowledgements ·
Chapter 3
3 Manuscript 2: Holocene vegetation and plant diversity changes in the north-eastern Siberian treeline region from pollen and sedimentary ancient DNA
3.1 Abstract
3.2 Introduction
3.3 Materials and methods
3.3.1 Study area
3.3.2 Lake sediment cores and subsampling
3.3.3 Dating
3.3.4 Pollen analysis
3.3.5 DNA extraction and amplification
3.3.6 Sequencing filtering and taxonomic assignment
3.3.7 Statistical analyses
3.4 Results
3.4.1 Chronology
3.4.2 SedaDNA and pollen assemblages
3.4.3 Gradient analysis and correlation analysis
3.5 Discussion
3 .5.1 Contributions of pollen and sedaDNA to vegetation reconstruction and taxon richness
3.5.2 Variation in Holocene vegetation composition in the Omoloy area, north-eastern Siberia
3.5.3 SedaDNA-based plant diversity changes within lake catchments of the Omoloy region
3.6 Conclusions
3.7 Acknowledgements
Chapter 4
4 Manuscript 3: Vegetation reconstruction from Siberia and Tibetan Plateau using modern analogue technique - comparing sedimentary ancient DNA (sedaDNA) and pollen data
4.1 Abstract
4.2 Introduction
4.3 Materials and methods
4.3.1 Sites ofthe modern analogues
4.3.2 Sedimentary (ancient) DNA collection
4.3.3 Metabarcoding data processing and filtering
4.3.4 Pollen data collection
4.3.5 Numerical analysis
4.4 Results
4.4.1 Modern training-set, ROC curve analyses and AT results
4.4.2 Modern analogues for Lake Naleng and Omoloy lake II
4.4.3 Vegetation type reconstruction based on MAT
4.4.4 Projecting fossil samples in ordination space of modern assemblages
4.4.5 Comparing past and present intertaxa relationships
4.5 Discussion
4.5.1 Assessment of analogue quality using modem training-sets
4·5·2 Comparison of sed(a)DNA-based and pollen-based vegetation reconstruction for the Lake Naleng, Tibetan Plateau
4.5.3 Comparison of sedDNA based and pollen-based vegetation reconstruction for the Lake Omoloy, northern Siberia
4.6 Conclusions
4.7 Acknowledgements
Chapter 5
5 Manuscript 4: Terrestrial-aquatic ecosystem links on the Tibetan Plateau inferred from sedaDNA shotgun sequencin
5.1 Abstract
5.2 Introduction
5.3 Results
5.4 Discussions
5.5 Methods
5.6 Acknowledgments
Chapter 6
6 Synthesis
6.1 The ability of metabarcoding and metagenomic shotgun sequencing to reveal ecological community pattern
6.2 Driver of plant diversity change in high altitude and high latitudes
6.3 High-altitude and high-latitude vegetation type change
6.4 Past terrestrial and aquatic ecological change at ecosystem-scale
6.5 Conclusions and outlook
Appendix
1 Appendix-1 Materials for Manuscript #1
1.1 Appendix discussion: Contamination in NTC6
2. Appendix-2 Materials for Manuscript #2
3. Appendix-3 Materials for Manuscript #3
4. Appendix-4 Materials for Manuscript #4
References
Eidesstattliche Erklarung
Language:
English
Keywords:
Hochschulschrift
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