Format:
Online-Ressource
ISSN:
1439-7633
Content:
Abstract: The synthesis and properties two series of new 2′‐O‐methyl RNA probes, each containing a single insertion of a 2′‐bispyrenylmethylphosphorodiamidate derivative of a nucleotide (U, C, A, and G), are described. As demonstrated by UV melting studies, the probes form stable complexes with model RNAs and DNAs. Significant increases (up to 21‐fold) in pyrene excimer fluorescence intensity were observed upon binding of most of the probes with complementary RNAs, but not with DNAs. The fluorescence spectra are independent of the nature of the modified nucleotides. The nucleotides on the 5′‐side of the modified nucleotide have no effect on the fluorescence spectra, whereas the natures of the two nucleotides on the 3′‐side are important: CC, CG, and UC dinucleotide units on the 3′‐side of the modified nucleotide provide the maximum increases in excimer fluorescence intensity. This study suggests that these 2′‐bispyrene‐labeled 2′‐O‐methyl RNA probes might be useful tools for detection of RNAs.
In:
volume:15
In:
number:13
In:
year:2014
In:
pages:1939-1946
In:
extent:8
In:
ChemBioChem, Weinheim : Wiley-VCH, [2000]-, 15, Heft 13 (2014), 1939-1946 (gesamt 8), 1439-7633
Language:
English
DOI:
10.1002/cbic.201402105
URN:
urn:nbn:de:101:1-2023011909071310088159
URL:
https://doi.org/10.1002/cbic.201402105
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023011909071310088159
URL:
https://d-nb.info/1278580123/34
URL:
https://doi.org/10.1002/cbic.201402105
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