Format:
Online-Ressource
ISSN:
1615-9861
Content:
Abstract: Efficient methods for profiling of the cell surface proteome are desirable to get a deeper insight in basic biological processes, to localise proteins and to uncover proteins differentially expressed in diseases. Here we present a strategy to target cell surface exposed proteins via fluorescence labelling using CyDye DIGE fluors. This method has been applied to human cell lines in vitro as well as to a complex biological system in vivo. It allows detection of fluorophore‐tagged cell surface proteins and visualisation of the accessible proteome within a single 2‐D gel, simplifying subsequent UV MALDI‐MS analysis.
In:
volume:6
In:
number:2
In:
year:2006
In:
pages:579-585
In:
extent:7
In:
Proteomics, Weinheim : Wiley VCH, [2001]-, 6, Heft 2 (2006), 579-585 (gesamt 7), 1615-9861
Language:
English
DOI:
10.1002/pmic.200500104
URN:
urn:nbn:de:101:1-2023090307011882909011
URL:
https://doi.org/10.1002/pmic.200500104
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023090307011882909011
URL:
https://d-nb.info/1300786337/34
URL:
https://doi.org/10.1002/pmic.200500104
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