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  • 1
    Online-Ressource
    Online-Ressource
    Hindawi Limited ; 2021
    In:  Evidence-Based Complementary and Alternative Medicine Vol. 2021 ( 2021-5-19), p. 1-13
    In: Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2021 ( 2021-5-19), p. 1-13
    Kurzfassung: Cynanchum paniculatum (Bge.) Kitag. (CP) is an important medicinal herb used in Chinese herbal medicine, with a variety of biological activities including anticancer property. In this study, we explored the water extract of CP, for its anticancer effects against breast cancer cells with different mutation types. Cells were grouped as untreated (Control); CP direct treatment (dir-CP); Conditioned medium from CP treated (sup-CP), and untreated cells (sup-Control). Effects of dir-CP and sup-CP were compared to corresponding untreated cells on cytotoxicity, cell migration, and protein expression (cleaved caspase-3, caspase-9, and MMP-2 and 9). CP treatment showed time-dependent decrease in cell number of MDA-MB-231 and SK-Br-3 (both ER(−) PR(−)), while the decrease in cell number was not as significant in MCF-7 and ZR-75-1 cells (both ER(+) PR(+)). sup-CP treatment inhibited the cell migration of MDA-MB-231 and MCF-7 (Her2(−)) in a 24 h scratch assay. Our data suggested that ER(−) PR(−) cells are more sensitive to the CP in terms of direct cytotoxicity, which is not regulated by caspase-3. CP inhibited the migration of the two Her2(−) cells, and this correlated with MMP-2 regulation. The migration of ER(−) PR(−) cells was more sensitive to conditioned medium with CP treatment than to direct CP, and this is not regulated by MMP-2. Our data suggested that CP has anticancer potential on various breast cancer cells through different mechanisms and is specifically effective in inhibiting the migration of the triple negative MDA-MB-231. Our data provide insight into the mechanism of CP against breast cancer progression and would benefit the medical practitioners in better management with CP usage.
    Materialart: Online-Ressource
    ISSN: 1741-4288 , 1741-427X
    Sprache: Englisch
    Verlag: Hindawi Limited
    Publikationsdatum: 2021
    ZDB Id: 2148302-4
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    In: Biomolecules, MDPI AG, Vol. 11, No. 2 ( 2021-01-29), p. 187-
    Kurzfassung: Kaempferitrin is extracted in significantly high quantities from the leaves of Cinnamomum osmophloeum, which belongs to a group of plant species that comes under the genus Cinnamomum, well-known for its established anti-diabetic property in Chinese medicine. Oral administration of kaempferitrin and Cinnamomum osmophloeum extract reduced blood sugar in alloxan-induced diabetic rats and improved the lipid profile in hamsters respectively. In this paper we studied the differential protein expression profile using mass spectrometry approach in the kaempferitrin-treated conditioned medium of liver cancer cell line HepG2. We discovered that 33 genes were up/down-regulated consistently between two biological samples. A slightly different version of the analysis software selected 28 genes, and the final 18 genes that appeared in both lists were selected. Interestingly, 5 proteins out of 18 were either exosomal markers or reported in high frequency of occurrence in exosome/secreted vesicles. We also examined the extracellular particles with atomic force microscopy (AFM), which showed that the conditioned medium of kaempferitrin treated had larger vesicles and fewer small vesicles. Expression of some lipid-regulating genes were also altered. Our data suggested that extracellular vesicle secretions may be regulated by kaempferitrin, and regulation of lipid profile by kampeferitrin involves multiple mechanisms.
    Materialart: Online-Ressource
    ISSN: 2218-273X
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2021
    ZDB Id: 2701262-1
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    In: The Plant Journal, Wiley, Vol. 77, No. 1 ( 2014-01), p. 1-15
    Kurzfassung: Arabidopsis AGL 13 is a member of the AGL 6 clade of the MADS box gene family. GUS activity was specifically detected from the initiation to maturation of both pollen and ovules in AGL 13: GUS Arabidopsis. The sterility of the flower with defective pollen and ovules was found in AGL 13 RNA i knockdown and AGL 13 +  SRDX dominant‐negative mutants. These results indicate that AGL 13 acts as an activator in regulation of early initiation and further development of pollen and ovules. The production of similar floral organ defects in the severe AGL 13 +  SRDX and SEP 2 +  SRDX plants and the similar enhancement of AG nuclear localization efficiency by AGL 13 and SEP 3 proteins suggest a similar function for AGL 13 and E functional SEP proteins. Additional fluorescence resonance energy transfer ( FRET ) analysis indicated that, similar to SEP proteins, AGL 13 is able to interact with AG to form quartet‐like complexes ( AGL 13– AG ) 2 and interact with AG – AP 3– PI to form a higher‐order heterotetrameric complex ( AGL 13– AG – AP 3– PI ). Through these complexes, AGL 13 and AG could regulate the expression of similar downstream genes involved in pollen morphogenesis, anther cell layer formation and the ovule development. AGL 13 also regulates AG / AP 3/ PI expression by positive regulatory feedback loops and suppresses its own expression through negative regulatory feedback loops by activating AGL 6 , which acts as a repressor of AGL 13 . Our data suggest that AGL 13 is likely a putative ancestor for the E functional genes which specifies male and female gametophyte morphogenesis in plants during evolution.
    Materialart: Online-Ressource
    ISSN: 0960-7412 , 1365-313X
    URL: Issue
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2014
    ZDB Id: 2020961-7
    SSG: 12
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Online-Ressource
    Online-Ressource
    Chaoyang University of Technology (IJASE) ; 2022
    In:  International Journal of Applied Science and Engineering Vol. 19, No. 1 ( 2022), p. 1-11
    In: International Journal of Applied Science and Engineering, Chaoyang University of Technology (IJASE), Vol. 19, No. 1 ( 2022), p. 1-11
    Materialart: Online-Ressource
    ISSN: 1727-2394 , 1727-7841
    Sprache: Englisch
    Verlag: Chaoyang University of Technology (IJASE)
    Publikationsdatum: 2022
    ZDB Id: 3108486-2
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  • 5
    In: Frontiers in Surgery, Frontiers Media SA, Vol. 9 ( 2022-4-28)
    Kurzfassung: We investigated factors associated with postoperative lipiduria and hypoxemia in patients undergoing surgery for orthopedic fractures. We enrolled patients who presented to our emergency department due to traumatic fractures between 2016 and 2017. We collected urine samples within 24 h after the patients had undergone surgery to determine the presence of lipiduria. Hypoxemia was defined as an SpO 2 & lt;95% determined with a pulse oximeter during the hospitalization. Patients’ anthropometric data, medical history, and laboratory test results were collected from the electronic medical record. Logistic regression analyses were used to determine the associations of clinical factors with postoperative lipiduria and hypoxemia with multivariate adjustments. A total of 144 patients were analyzed (mean age 51.3 ± 22.9 years, male 50.7%). Diabetes (odd ratio 3.684, 95% CI, 1.256–10.810, p  = 0.018) and operation time (odd ratio 1.005, 95% CI, 1.000–1.009, p  = 0.029) were independently associated with postoperative lipiduria, while age (odd ratio 1.034, 95% CI, 1.003–1.066, p  = 0.029), body mass index (odd ratio 1.100, 95% CI, 1.007–1.203, p  = 0.035), and operation time (odd ratio 1.005, 95% CI, 1.000–1.010, p  = 0.033) were independently associated with postoperative hypoxemia. We identified several factors independently associated with postoperative lipiduria and hypoxemia in patients with fracture undergoing surgical intervention. Operation time was associated with both postoperative lipiduria and hypoxemia, and we recommend that patients with prolonged operation for fractures should be carefully monitored for clinical signs related to fat embolism syndrome.
    Materialart: Online-Ressource
    ISSN: 2296-875X
    Sprache: Unbekannt
    Verlag: Frontiers Media SA
    Publikationsdatum: 2022
    ZDB Id: 2773823-1
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 6
    In: The Plant Journal, Wiley, Vol. 105, No. 5 ( 2021-03), p. 1357-1373
    Kurzfassung: We developed a strategy using in vivo fluorescence resonance energy transfer (FRET) based on the distance change and distance symmetry of a stable tetrameric complex in tobacco ( Nicotiana benthamiana ) leaf cells to improve the accuracy of the estimation of heterotetrameric complex formation. This strategy was applied successfully to show that various heterotetrameric complexes containing homodimers or heterodimers of B function proteins coexist in the regulation of tepal/stamen formation in lily ( Lilium longiflorum ).
    Materialart: Online-Ressource
    ISSN: 0960-7412 , 1365-313X
    URL: Issue
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2021
    ZDB Id: 2020961-7
    SSG: 12
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 7
    Online-Ressource
    Online-Ressource
    MDPI AG ; 2021
    In:  International Journal of Environmental Research and Public Health Vol. 18, No. 17 ( 2021-09-06), p. 9386-
    In: International Journal of Environmental Research and Public Health, MDPI AG, Vol. 18, No. 17 ( 2021-09-06), p. 9386-
    Kurzfassung: Background: The waiting time (WT) for a phlebotomy is directly related to patient satisfaction with a health service. However, the processing time varies widely depending on the type of patients. Monitoring of the WT alone may not enable an effective evaluation of the lean performance of the medical staff for patients with different characteristics. The objective of this study was to use process cycle efficiency (PCE) to assess the performance of an intelligent tube preparation system (ITPS) which automatically labeled test tubes and conducted patient rerouting for phlebotomy services, and to interpret the WT during peak hours. Methods: Three time periods were used. The baseline period was from 1 July to 31 July 2014. Phase 1 was after the establishment of the ITPS, with patients ≥80 years old being rerouted. In phase 2, patients ≥78 years old were rerouted. Those data were recorded with a calling system and ITPS, respectively. Results: PCE was significantly improved from 12.9% at baseline to 51.1% (p 〈 0.001) in phase 1 and 53.0% (p 〈 0.001) in phase 2. The WT of 16.9 min at baseline was reduced to 3.8 min in phase 1 (p 〈 0.001), and 3.6 min in phase 2 (p 〈 0.001). Moreover, the results showed that a WT 〈 10 min was consistent with a PCE ≥ 25%. Conclusions: Establishing an ITPS for phlebotomy can significantly increase PCE and shorten the WT. Furthermore, the PCE ≥ 25% could be a good assessment reference for the management of appropriate human resources for phlebotomy services, although it is a complex parameter.
    Materialart: Online-Ressource
    ISSN: 1660-4601
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2021
    ZDB Id: 2175195-X
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 8
    In: Diabetes, American Diabetes Association, Vol. 57, No. 7 ( 2008-07-01), p. 1814-1823
    Kurzfassung: OBJECTIVE—Estradiol (E2) is known to modulate insulin sensitivity and, consequently, glucose homeostasis. Resveratrol (RSV), an agonist of estrogen receptor (ER), has exerted antihyperglycemic effects in streptozotocin-induced type 1 diabetic rats in our previous study and was also shown to improve insulin resistance in other reports. However, it remains unknown whether activation of ER is involved in the metabolic effects of RSV via insulin-dependent and -independent mechanisms. RESEARCH DESIGN AND METHODS—Male Sprague-Dawley rats were given a high cholesterol–fructose (HCF) diet for 15 weeks and were treated with RSV for either 15 days or 15 weeks. RESULTS—Here, we show that RSV shifts the metabolic characteristics of rats on an HCF diet toward those of rats on a standard diet. RSV treatment increased insulin-stimulated whole-body glucose uptake and steady-state glucose uptake of soleus muscle and liver in HCF-fed rats as well as enhanced membrane trafficking activity of GLUT4 and increased phosphorylation of insulin receptor in insulin-resistant soleus muscles. Interestingly, the phosphorylated ER level in insulin-resistant soleus muscle was significantly elevated in rats with RSV treatment in both basal and euglycemic-hyperinsulinemic conditions. RSV exerted an insulin-like stimulatory effect on isolated soleus muscle, epididymal fat and hepatic tissue, and C2C12 myotubes. The RSV-stimulated glucose uptake in C2C12 myotubes was dependent on extracellular signal–related kinase/p38 (early phase, 1 h) and p38/phosphoinositide 3-kinase (late phase, 14 h) activation. Inhibition of ER abrogated RSV-induced glucose uptake in both early and late phases. CONCLUSIONS—Collectively, these results indicate that ER is a key regulator in RSV-stimulating insulin-dependent and -independent glucose uptake, which might account for the protective effects of RSV on diet-induced insulin resistance syndrome.
    Materialart: Online-Ressource
    ISSN: 0012-1797 , 1939-327X
    Sprache: Englisch
    Verlag: American Diabetes Association
    Publikationsdatum: 2008
    ZDB Id: 1501252-9
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 9
    Online-Ressource
    Online-Ressource
    MDPI AG ; 2020
    In:  Nutrients Vol. 12, No. 11 ( 2020-11-18), p. 3535-
    In: Nutrients, MDPI AG, Vol. 12, No. 11 ( 2020-11-18), p. 3535-
    Kurzfassung: Melanogenesis has many important physiological functions. However, abnormal melanin production causes various pigmentation disorders. Melanin synthesis is stimulated by α-melanocyte stimulating hormone (α-MSH) and ultraviolet (UV) irradiation. Lotus seedpod extract (LSE) has been reported as possessing antioxidative, anti-aging, and anticancer activities. The present study examined the effect of LSE on melanogenesis and the involved signaling pathways in vitro and in vivo. Results showed that non-cytotoxic doses of LSE and its main component epigallocatechin (EGC) reduced both tyrosinase activity and melanin production in the α-MSH-induced melanoma cells. Western blotting data revealed that LSE and EGC inhibited expressions of tyrosinase and tyrosinase-related protein 1 (TRP-1). Phosphorylation of p38 and protein kinase A (PKA) stimulated by α-MSH was efficiently blocked by LSE treatment. Furthermore, LSE suppressed the nuclear level of cAMP-response element binding protein (CREB) and disturbed the activation of melanocyte inducing transcription factor (MITF) in the α-MSH-stimulated B16F0 cells. The in vivo study revealed that LSE inhibited melanin production in the ear skin of C57BL/6 mice after exposure to UVB. These findings suggested that the anti-melanogenesis of LSE involved both PKA and p38 signaling pathways. LSE is a potent novo natural depigmenting agent for cosmetics or pharmaceutical applications.
    Materialart: Online-Ressource
    ISSN: 2072-6643
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2020
    ZDB Id: 2518386-2
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 10
    Online-Ressource
    Online-Ressource
    Springer Science and Business Media LLC ; 2009
    In:  Journal of Biomedical Science Vol. 16, No. 1 ( 2009-12)
    In: Journal of Biomedical Science, Springer Science and Business Media LLC, Vol. 16, No. 1 ( 2009-12)
    Kurzfassung: In this study, we established systemic in-vivo evidence from molecular to organism level to explain how diabetes can aggravate myocardial ischemia-reperfusion (I/R) injury and revealed the role of insulin signaling (with specific focus on Akt/GLUT4 signaling molecules). The myocardial I/R injury was induced by the left main coronary artery occlusion for 1 hr and then 3 hr reperfusion in control, streptozotocin (STZ)-induced insulinopenic diabetes, and insulin-treated diabetic rats. The diabetic rats showed a significant decrease in heart rate, and a prolonged isovolumic relaxation (tau) which lead to decrease in cardiac output (CO) without changing total peripheral resistance (TPR). The phosphorylated Akt and glucose transporter 4 (GLUT 4) protein levels were dramatically reduced in both I/R and non-I/R diabetic rat hearts. Insulin treatment in diabetes showed improvement of contractile function as well as partially increased Akt phosphorylation and GLUT 4 protein levels. In the animals subjected to I/R, the mortality rates were 25%, 65%, and 33% in the control, diabetic, and insulin-treated diabetic group respectively. The I/R-induced arrhythmias and myocardial infarction did not differ significantly between the control and the diabetic groups. Consistent with its anti-hyperglycemic effects, insulin significantly reduced I/R-induced arrhythmias but had no effect on I/R-induced infarctions. Diabetic rat with I/R exhibited the worse hemodynamic outcome, which included systolic and diastolic dysfunctions. Insulin treatment only partially improved diastolic functions and elevated P-Akt and GLUT 4 protein levels. Our results indicate that cardiac contractile dysfunction caused by a defect in insulin-stimulated Akt/GLUT4 may be a major reason for the high mortality rate in I/R injured diabetic rats.
    Materialart: Online-Ressource
    ISSN: 1423-0127
    Sprache: Englisch
    Verlag: Springer Science and Business Media LLC
    Publikationsdatum: 2009
    ZDB Id: 1482918-6
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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