In:
Current Microbiology, Springer Science and Business Media LLC, Vol. 79, No. 8 ( 2022-08)
Abstract:
Cream colored bacteria from marine agar, strain WH24, WH77, and WH80 were isolated from the gill of the Crassostrea gigas a Pacific oyster with a filter-feeding habit that compels accompanying bacteria to demonstrate a high metabolic capacity, has proven able to colonize locations with changing circumstances. Based on the 16S rRNA gene sequence, all strains had high similarity to Photobacterium arenosum CAU 1568 T (99.72%). This study involved phenotypic traits, phylogenetic analysis, antimicrobial activity evaluation, genome mining, Co-cultivation experiments, and chemical studies of crude extracts using HPLC and LC-HRESIMS. Photobacterium arenosum WH24 and Zooshikella harenae WH53 T were co-cultivated for 3 days in a rotary shaker at 160 rpm at 30 °C, and LC-MS monitored the chemical profiles of the co-cultures on the third day. The UV chromatograms of the extracts of the co-cultivation experiments show that Zooshikella harenae WH53 T could be inhibited by strain WH24. The high virulence of Photobacterium arenosum WH24 was confirmed by genome analysis. Gene groups with high virulence potential were detected: tss A ( Imp A), tss B ( Imp B/ vip A), tss C ( Imp C/ vip B), tss E, tss F ( Imp G/ vas A), tss G ( Imp H/vasB), tss M ( Icm F/ vas K), tss J ( vas D), tss K ( Imp J/ vas E), tss L ( Imp K/ vas F), clp V ( tss H), vas H, hcp , lap P, plp D, and tps B family.
Type of Medium:
Online Resource
ISSN:
0343-8651
,
1432-0991
DOI:
10.1007/s00284-022-02909-2
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2022
detail.hit.zdb_id:
1458987-4
SSG:
12
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