In:
European Journal of Immunology, Wiley, Vol. 40, No. 5 ( 2010-05), p. 1504-1509
Kurzfassung:
HIV replication is restricted by some anti‐CD4 mouse mAb in vitro and in vivo . However, a human monoclonal anti‐CD4 Ab has not been isolated. We screened EBV‐transformed peripheral B cells from 12 adult donors for CD4‐reactive Ab production followed by functional reconstitution of Fab genes. Three independent IgM Fab clones reactive specifically to CD4 were isolated from a healthy HIV‐seronegative adult (∼0.0013% of the peripheral B cells). The germ line combinations for the V H and V L genes were V H 3‐33/ L 6, V H 3‐33/ L 12, and V H 4‐4/ L 12, respectively, accompanied by somatic hypermutations. Genetic analysis revealed a preference for V‐gene usage to develop CD4‐reactive Ab. Notably, one of the CD4‐reactive clones, HO538‐213, with an 1×10 −8 M dissociation constant (Kd) to recombinant human CD4, limited the replication of R5‐tropic and X4‐tropic HIV‐1 strains at 1–2.5 μg/mL in primary mononuclear cells. This is the first clonal genetic analysis of human monoclonal CD4‐reactive Ab. A mAb against CD4 isolated from a healthy individual could be useful in the intervention of HIV/AIDS.
Materialart:
Online-Ressource
ISSN:
0014-2980
,
1521-4141
DOI:
10.1002/eji.200939479
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2010
ZDB Id:
1491907-2
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