In:
Frontiers in Microbiology, Frontiers Media SA, Vol. 12 ( 2021-4-6)
Kurzfassung:
The coffee berry borer, the most economically important insect pest of coffee worldwide, is the only insect capable of feeding and reproducing solely on the coffee seed, a food source containing the purine alkaloid caffeine. Twenty-one bacterial species associated with coffee berry borers from Hawai’i, Mexico, or a laboratory colony in Maryland ( Acinetobacter sp. S40, S54, S55, Bacillus aryabhattai , Delftia lacustris , Erwinia sp. S38, S43, S63, Klebsiella oxytoca , Ochrobactrum sp. S45, S46, Pantoea sp. S61, Pseudomonas aeruginosa , P. parafulva , and Pseudomonas sp. S30, S31, S32, S37, S44, S60, S75) were found to have at least one of five caffeine N-demethylation genes ( ndmA , ndmB , ndmC , ndmD , ndmE ), with Pseudomonas spp. S31, S32, S37, S60 and P . parafulva having the full complement of these genes. Some of the bacteria carrying the ndm genes were detected in eggs, suggesting possible vertical transmission, while presence of caffeine-degrading bacteria in frass, e.g., P. parafulva ( ndmABCDE ) and Bacillus aryabhattai ( ndmA ) could result in horizontal transmission to all insect life stages. Thirty-five bacterial species associated with the insect ( Acinetobacter sp. S40, S54, S55, B. aryabhattai , B. cereus group, Bacillus sp. S29, S70, S71, S72, S73, D. lacustris , Erwinia sp. S38, S43, S59, S63, K. oxytoca , Kosakonia cowanii , Ochrobactrum sp. S45, S46, Paenibacillus sp. S28, Pantoea sp. S61, S62, P. aeruginosa , P. parafulva , Pseudomonas sp. S30, S31, S32, S37, S44, S60, S75, Stenotrophomonas sp. S39, S41, S48, S49) might contribute to caffeine breakdown using the C-8 oxidation pathway, based on presence of genes required for this pathway. It is possible that caffeine-degrading bacteria associated with the coffee berry borer originated as epiphytes and endophytes in the coffee plant microbiota.
Materialart:
Online-Ressource
ISSN:
1664-302X
DOI:
10.3389/fmicb.2021.644768
DOI:
10.3389/fmicb.2021.644768.s001
DOI:
10.3389/fmicb.2021.644768.s002
DOI:
10.3389/fmicb.2021.644768.s003
DOI:
10.3389/fmicb.2021.644768.s004
Sprache:
Unbekannt
Verlag:
Frontiers Media SA
Publikationsdatum:
2021
ZDB Id:
2587354-4
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