In:
Kidney and Blood Pressure Research, S. Karger AG, Vol. 44, No. 6 ( 2019), p. 1339-1351
Kurzfassung:
〈 b 〉 〈 i 〉 Aim: 〈 /i 〉 〈 /b 〉 This study aimed to investigate the effect of norcantharidin (NCTD) on human mesangial cells (HMCs) apoptosis in vitro and further examine its molecular mechanism. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 HMCs were divided into 5 groups: control group, 25% fetal bovine serum (FBS)-treated group, and NCTD groups (NCTD [2.5, 5 and 10 µg/mL] + 25% FBS, respectively). Cell proliferation was determined by MTT assay, while apoptosis was evaluated by Hoechest 33258 staining, the level of cytochrome c, immunohistochemistry, and apoptotic-related proteins/gene expression. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 Cell viability was inhibited in NCTD-treated HMCs in a dose-dependent manner. The number of apoptotic cells and the content of cytochrome c were significantly increased by NCTD treatment but that of mitochondrial membrane was decreased. Moreover, the expression of bcl-2 and caspase-3 was prompted by NCTD, but the expression of bax, MMP-2, and MMP-9 in 25% FBS-treated HMCs was inhibited. In addition, NCTD markedly unregulated the expression of apoptosis-related gene/protein, including p-Erk1/2, phosphorylated-Jun N-terminal kinase (JNK), p-p38, and p53. 〈 b 〉 〈 i 〉 Conclusion: 〈 /i 〉 〈 /b 〉 NCTD enhances 25% FBS-treated HMC apoptosis in vitro, and this effect may be attributed to the modulation of the ERK, JNK, and p38 mitogen-activated protein kinase signaling pathways.
Materialart:
Online-Ressource
ISSN:
1420-4096
,
1423-0143
Sprache:
Englisch
Verlag:
S. Karger AG
Publikationsdatum:
2019
ZDB Id:
1482922-8
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